响应表面的优化,净化,表征和从Thermomyces dupontii的酸性,热稳定的基因酶中产生短链基因氧糖糖化合物
Rajni Kumari1, Manish Kumar1, Pawan K Dadheech1
1Department of Microbiology, School of Life Sciences, Central University of Rajasthan Bandarsindri, Kishangarh, Ajmer 305801, Rajasthan, India.
International journal of biological macromolecules
|April 7, 2024
概括
这项研究优化了一种新型真菌的奇丁酶生产,产生一种酸性,热稳定的酶. 这种酶有效地分解了基,为废物利用提供了环保的解决方案.
科学领域:
- 生物技术和工业微生物学
- 酶学 是一种酶学.
- 生物催化剂是一种生物催化剂.
背景情况:
- 在沿海废物中丰富的基,为食品和生物技术行业提供了可持续的资源.
- 传统的酸盐加工依赖于强化学品,需要更绿色的替代品.
- 与化学方法相比,酶方法提供了更好的控制和产品均性.
研究的目的:
- 为了优化一种新型热友真菌菌株,Thermomyces dupontii,ITCC 9104.4的基因酶生产.
- 为潜在的工业应用净化和特征化一种酸性,耐热稳定的基因酶 (TdChiT).
- 为了评估酶的活性,稳定性和基质特异性,以有效地增值素.
主要方法:
- 优化了过程变量,以提高来自*Thermomyces dupontii*的奇丁酶生产率.
- 净化了一种酸性,热稳定的基因酶 (TdChiT),并确定其分子质量.
- 评估了酶活性,不同温度和pH值的稳定性以及基质特异性.
- 研究了金属离子和表面活性剂对酶活性的影响.
主要成果:
- 在优化条件下 (96小时,45°C) 达到3.8809个单位/升/小时的最大胆酶生产率.
- 净化了60kDa的酸性,热稳定的奇他酶 (TdChiT),在70°C和pH 5.0时具有最佳活性.
- 在脱糖化后,TdChiT的活性降低,这表明糖化对其稳定性的作用.
- 该酶表现出对体素和 (GlcNAc) 2-4的特异性,在某些金属离子和非离子表面活性剂的存在下增强活性.
结论:
- 从*Thermomyces dupontii*中优化TdChiT的生产提供了一个可持续的生物催化剂.
- 描述的酸性,耐热稳定的基因酶显示了对基因废物的环保回收的潜力.
- TdChiT可以促进资源高效的工业过程,将类废物转化为增值产品.
相关概念视频
01:25Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
01:30Production of Pharmaceuticals
99
Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under...
99
01:24iChip
107
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
107