使用纸喷雾质谱仪进行等离子体药物查,并集成固相提取
Hannah Zimmerman-Federle1, Greta Ren1, Sarah Dowling1
1Department of Chemistry and Chemical Biology, Indiana University-Purdue University Indianapolis, Indianapolis, Indiana, USA.
Drug testing and analysis
|April 8, 2024
概括
一种新方法使用纸质喷雾盒快速选约160种药物,包括芬太尼类同类药物和合成大麻素,在过量样本中. 这种技术在血中达到每毫升纳米克以下的检测极限,用于增强过量药物分析.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 随着药物过量率的上升,需要快速而敏感的分析方法.
- 现有的方法可能缺乏对新型精神活性物质进行全面查的速度或灵敏度.
- 紧急部门的样本对药物分析具有独特的挑战,原因是矩阵的复杂性和紧急的周转时间.
研究的目的:
- 开发和验证一种简单的,非向的方法来选大约160种药物过量样本中的药物.
- 通过纸张喷雾-液体色谱-质谱法,在血中达到每毫升亚纳米克对合成药物的敏感性.
- 为了在临床环境中快速识别新兴合成药物,包括芬太尼类同类药物和合成大麻素.
主要方法:
- 使用了一次性纸张喷雾盒,集成固体相提取 (SPE) 用于样品制备.
- 分析了来自急诊室的300多个真实过量样本,使用四极轨道飞行质谱学.
- 采用连续窗口获取所有理论碎片离子光谱 (SWATH) 与合质谱学.
主要成果:
- 在血中达到低检测极限 (LOD):合成大麻素0.10.5 ng/mL,芬太尼和类似物0.10.3 ng/mL.
- 构建了35种经常遇到的药物的同位素标记内部标准的校准曲线.
- 在真实的过量剂量样本上验证了该方法,检测出常见的合成药物,如芬太尼相关物质,设计型二胺 (例如,flubromazolam) 和5F-PB-22.
结论:
- 开发的纸喷雾SPE方法是一种简单,快速和灵敏的工具,用于过量服用病例的非向药物查.
- 该方法在检测临床相关度的新兴合成药物方面表现出色.
- 这种方法可以帮助急诊室及时诊断和治疗药物过量患者.
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