新型位点与葡萄藤中对黄和粉状菌的抗性有关
Valentina Ricciardi1, Manna Crespan2, Giuliana Maddalena1
1Dipartimento di Scienze Agrarie ed Ambientali, Università degli Studi di Milano, Milano, Italy.
Frontiers in plant science
|April 8, 2024
概括
这项研究确定了新的遗传基因因位点,用于葡萄树中菌 (DM) 和粉状菌 (PM) 耐药性. 这些发现支持了可持续葡萄种植和减少杀菌剂使用的育种努力.
科学领域:
- 植物遗传学和育种.
- 葡萄种植和葡萄酒学
- 农业的可持续性 农业的可持续性
背景情况:
- 葡萄种植面临着可持续的真菌病管理方面的挑战,特别是菌 (DM) 和粉状菌 (PM).
- 育种耐病葡萄品种对于减少杀菌剂的应用至关重要.
- 探索葡萄酒的遗传多样性是识别耐药性特征的关键.
研究的目的:
- 从高加索生殖质中识别出抗DM和PM的葡萄藤基因型.
- 使用全基因组关联分析 (GWAS) 发现与DM和PM耐药性相关的新型遗传位置.
- 为开发抗病葡萄酒品种提供见解.
主要方法:
- 使用13个简单序列重复 (SSR) 位点对88个葡萄藤基因型 (栽培和野生) 的分析.
- 通过人工注射DM和PM病原体进行表型评估.
- 全基因组关联分析 (GWAS) 用单核酸多态性 (SNP) 资料来识别耐药性位置.
主要成果:
- 在SSR分析中,16种基因型中检测出了耐药性等位基因.
- 现型查发现了7种耐DM和31种耐PM的基因型.
- 在GWAS中,发现了两个DM耐药性的新基点 (染色体15和16上的Rpv36,Rpv37) 和两个PM耐药性的新基点 (染色体6和17上的Ren14,Ren15).
结论:
- 已识别的基因位置位于富含生物应激反应的基因的基因区域.
- 这项研究提供了有价值的候选基因和基因位点,用于培育抗病葡萄.
- 建议对这些位点进行进一步的功能性表征,以优化它们在育种计划中的使用.
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