双色四相全场光学连贯显微镜双色四相全场光学连贯显微镜
Rishyashring R Iyer1,2, Mantas Žurauskas1, Yug Rao1,2
1University of Illinois Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Urbana, Illinois, United States.
Journal of biomedical optics
|April 8, 2024
概括
双色四相 (BiTe) 全场光学连贯显微镜 (FF-OCM) 提供动态强度,相位和光谱对比成像,无需移动部件. 这种先进的FF-OCM技术最大限度地提高了生物样本表征的信息效率.
科学领域:
- 生物医学光学 生物医学光学
- 显微镜技术 显微镜技术
- 频谱学是一种光谱学.
背景情况:
- 全场光学连贯显微镜 (FF-OCM) 广泛用于反向散射和相位成像.
- 传统的FF-OCM方法受到功能信息利用的限制和复杂的光学设计,其移动部分用于相位对比.
研究的目的:
- 引入一种新的FF-OCM设置,双色四相 (BiTe) FF-OCM,用于动态强度,相位和伪光谱对比成像.
- 为了实现单拍,全场,视频速率的成像,没有任何移动部件.
主要方法:
- BiTe FF-OCM设置使用额定带宽之外的反射涂层来产生四个独特的相位转移.
- 这些相位移被检测使用两个排放过器,使得光谱对比生成.
- 该系统可以捕捉动态强度和相位的形状,而不会有工件或斑点噪声.
主要成果:
- BiTe FF-OCM成功地在三维 (3D) 中成像了散射样品.
- 该技术产生了三个互补的对比:强度,相位和颜色.
- 展示的应用包括观察细胞动力学,在3D中成像活的微生物,捕捉组织的光谱血动力学,以及具有多色能力的成像微观结构.
结论:
- BiTe FF-OCM提高了FF-OCM中的信息效率.
- 设计简单,方便生物样品的定量,动态和光谱表征.
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