Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.2K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

8.0K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
8.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Label-free multimodal nonlinear microscopy enabled by an optical parametric generator.

APL photonics·2026
Same author

Label-free correlative morpho-chemical tomography of 3D kidney mesangial cells.

Journal of biomedical optics·2026
Same author

Unified Vibrational and Multiphoton Label-Free Nonlinear Microscopy for Simultaneous Chemical and Structural Imaging.

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same author

Shape-conformal porous frameworks for full coverage of neural organoids and high-resolution electrophysiology.

Nature biomedical engineering·2026
Same author

Corrections to "Nonlinear Imaging Histopathology: A Pipeline to Correlate Gold-Standard Hematoxylin and Eosin Staining With Modern Nonlinear Microscopy".

IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society·2026
Same author

Glutamate indicators with increased sensitivity and tailored deactivation rates.

Nature methods·2025

相关实验视频

Updated: Jun 29, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K

双色四相全场光学连贯显微镜双色四相全场光学连贯显微镜

Rishyashring R Iyer1,2, Mantas Žurauskas1, Yug Rao1,2

  • 1University of Illinois Urbana-Champaign, Beckman Institute for Advanced Science and Technology, Urbana, Illinois, United States.

Journal of biomedical optics
|April 8, 2024
PubMed
概括

双色四相 (BiTe) 全场光学连贯显微镜 (FF-OCM) 提供动态强度,相位和光谱对比成像,无需移动部件. 这种先进的FF-OCM技术最大限度地提高了生物样本表征的信息效率.

关键词:
生物动力学 生物动力学光学连贯性显微镜阶段成像成像技术的相位成像技术.谱光成像技术的成像

更多相关视频

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

1.6K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.5K

相关实验视频

Last Updated: Jun 29, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.3K
Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
07:51

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts

Published on: October 21, 2022

1.6K
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
11:21

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

11.5K

科学领域:

  • 生物医学光学 生物医学光学
  • 显微镜技术 显微镜技术
  • 频谱学是一种光谱学.

背景情况:

  • 全场光学连贯显微镜 (FF-OCM) 广泛用于反向散射和相位成像.
  • 传统的FF-OCM方法受到功能信息利用的限制和复杂的光学设计,其移动部分用于相位对比.

研究的目的:

  • 引入一种新的FF-OCM设置,双色四相 (BiTe) FF-OCM,用于动态强度,相位和伪光谱对比成像.
  • 为了实现单拍,全场,视频速率的成像,没有任何移动部件.

主要方法:

  • BiTe FF-OCM设置使用额定带宽之外的反射涂层来产生四个独特的相位转移.
  • 这些相位移被检测使用两个排放过器,使得光谱对比生成.
  • 该系统可以捕捉动态强度和相位的形状,而不会有工件或斑点噪声.

主要成果:

  • BiTe FF-OCM成功地在三维 (3D) 中成像了散射样品.
  • 该技术产生了三个互补的对比:强度,相位和颜色.
  • 展示的应用包括观察细胞动力学,在3D中成像活的微生物,捕捉组织的光谱血动力学,以及具有多色能力的成像微观结构.

结论:

  • BiTe FF-OCM提高了FF-OCM中的信息效率.
  • 设计简单,方便生物样品的定量,动态和光谱表征.