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从毒症患者的阳性标记血液培养中对抗菌素直接敏感性测试的评估

Vikramjeet Singh¹, Jyotsna Agarwal¹, Soumya S Nath²

¹Department of Microbiology, Dr. Ram Manohar Lohia Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.

Indian journal of critical care medicine : peer-reviewed, official publication of Indian Society of Critical Care Medicine

|April 8, 2024

概括

来自阳性血液培养的直接抗微生物敏感性测试 (AST) 显著减少了报告时间,长达24小时. 这种方法保持了准确性,帮助临床医生及时做出败血症治疗决定.

科学领域:

临床微生物学临床微生物学
传染性疾病传染性疾病
诊断测试诊断测试诊断测试诊断测试诊断测试

背景情况:

自动化抗微生物敏感性测试 (AST) 系统对于及时治疗败血症至关重要.
准确和快速的AST结果使临床医生能够选择合适的抗菌剂.
目前的方法往往涉及报告易感性结果的延迟.

研究的目的:

为了评估从阳性血液培养中直接AST (DAST) 的性能.
与标准参考AST (RAST) 相比,评估DAST的准确性和报告时间.
确定DAST在败血症管理中的临床实用性.

主要方法:

一项为期12个月的北印度重症监护室的研究,涉及200个阳性血液培养物.
使用MALDI-TOF-MS从阳性血液培养中直接识别细菌.
直接从中进行DAST,结果与使用VITEK-2的RAST进行比较.

主要成果:

对于肠道细菌 (99%对于大肠杆菌,肺炎) 和 Pseudomonas aeruginosa (99.6%) 的高分类协议.
对Acinetobacter spp.的良好协议 (95-96%) 是一个很好的协议. 和其他格拉姆阴性细菌.
DAST 减少了 18-24 小时的报告时间,无论是 Gram 阴性还是 Gram 阳性细菌.

结论:

来自阳性血液培养的直接AST显著缩短了细菌敏感性报告的时间.
在不影响准确性的情况下,DAST提供了相当大的时间优势 (长达24小时).
这种快速报告有助于更早,更有效地为败血症患者提供抗菌疗法.

关键词:

分类协议协议分类协议.直接的抗微生物敏感性测试.进入细菌体中的细菌.基本协议基本协议标志着血液培养的标志格拉姆阳性细菌是什么?非发酵剂的非发酵剂发布报告时间发布报告时间

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