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miR-145-5p和hsa-let-7a-3p对结肠直肠瘤发生的同时作用:体外证据

Nazila Mozammel¹, Elham Baghbani², Mohammad Amini²

¹Department of Biology, Higher Education Institute of Rab-Rashid, Tabriz, Iran.

Advanced pharmaceutical bulletin

|April 8, 2024

概括

替换瘤抑制器微RNA let-7和MIR-145一起显著降低了结直肠癌细胞的增殖和增加了亡. 这种联合miRNA疗法显示出作为结直肠癌 (CRC) 新治疗策略的前景.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
基因规则基因规则

背景情况:

微RNAs (miRNAs) 是调节性RNAs,涉及到癌症的发展.
let-7和MIR-145是结肠直肠癌 (CRC) 下调的瘤抑制剂.
这些miRNAs的失调有助于CRC进展.

研究的目的:

在CRC细胞中研究let-7和MIR-145替代的联合治疗效果.
评估对细胞增殖,细胞亡和迁移的影响.
评估关键癌症相关基因的调制.

主要方法:

使用了具有低let-7a-3p和MIR-145-5p表达的HCT-116CRC细胞.
细胞被hsa-let-7a和MIR-145单独和组合转染.
试验包括MTT用于生命力,流细胞计用于细胞亡和细胞循环,以及qRT-PCR用于基因表达.

主要成果:

同时表达hsa-let-7a和MIR-145降低了CRC细胞增殖,并诱导G1细胞周期停止.
联合传染通过调节Bax,Bcl-2,P53和caspase表达,显著增强了亡诱导.
这种miRNA组合更有效地降低了转移相关基因MMP-9和MMP-2的下调.

结论:

MIR-145和hsa-let-7a的外源性过度表达在CRC细胞中表现出合作性抗癌效应.
这些miRNAs的同时替换显示出显著的治疗潜力.
使用let-7和MIR-145的组合疗法可能是治疗CRC的新策略.

关键词:

结肠直肠癌是一种癌症.这就是MIR-145-5p.这是一个hsa-let-7a-3pp.

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