相关实验视频

Updated: May 5, 2026

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria

Published on: September 18, 2014

四极极,高极化染料:排放依赖粘度和选择性线粒体染色

Mariusz Tasior¹, Olena Vakuliuk¹, Antoni Wrzosek²

¹Institute of Organic Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.

ACS organic & inorganic Au

|April 8, 2024

概括

四极A-D-A型的1,4-二二[3,2-b] (DHPPs) 与和基团显示可调节的发射. 这些化合物是光显微镜的优秀粘度探针,特别是在生物成像中.

科学领域:

有机化学有机化学
材料科学材料科学材料科学
生物物理学的生物物理.

背景情况:

AD-A型分子,特别是1,4-二二[3,2-b] (DHPPs),正在研究它们的光物理性质.
研究了电子丰富的核和缺电子的替代物之间的电子通信的作用,以提高排放.

研究的目的:

用和替代剂合成和表征四极AD-A型DHPPs.
调查粘度对这些DHPPs的排放特性的影响.
评估这些DHPP作为光显微镜中的粘度探针的潜力.

主要方法:

合成DHPP衍生物与和基团.
光物理测量,包括在各种极性溶剂中测量辐射强度和光寿命.
对H9C2心肌细胞进行光成像,以证明粘度感应能力.

主要成果:

含有和替代物的DHPPs在500-600nm范围内表现出排放.
核心和替代物之间的增强电子通信对于粘性介质中的排放增强至关重要.
一种特定的DHPP衍生品显示光学亮度显著增加 (34,000在糖醇中vs700在MeOH中),粘度增加.

结论:

四极式DHPP是开发敏感粘度探测器的有希望的候选人.
观察到的依赖粘度的光行为使它们适合在光显微镜中的应用.
在成像心肌细胞的成功演示突出了它们对生物传感应用的潜力.

更多相关视频

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy

Published on: June 4, 2020

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome

Published on: November 30, 2022

相关概念视频

Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...