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卵细胞线粒体将母体环境与后代的表型联系起来
Jason F Cooper1, Kim Nguyen1, Darrick Gates1
1Van Andel Research Institute, Department of Metabolism and Nutritional Programing, Grand Rapids, Michigan, USA, 49503.
Research square
|April 8, 2024
概括
通过胰岛素信号调节的母卵细胞线粒体重塑,增强了后代的应激适应能力. 这个过程取决于卵细胞电子运输链 (ETC) 的组成,影响后代的新陈代谢和生存.
科学领域:
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
- 代谢过程中的代谢.
背景情况:
- 卵子细胞在成熟过程中经历线粒体蛋白质组重塑,涉及电子输送链 (ETC) 子单元的变化,由母体胰岛素信号调节.
- 卵细胞线粒体重塑的适应意义尚不清楚,假设表明它在保护反应性氧物种 (ROS) 中起作用.
- 代际应激适应,就像在C. elegans*中母亲透应激暴露后增强后代的存活率一样,也通过卵细胞胰岛素信号传递进行介导.
研究的目的:
- 研究母体胰岛素信号和卵细胞线粒体ETC组成在后代对未来压力的适应性反应中的作用.
- 阐明将母体环境与后代代谢适应联系起来的分子机制.
主要方法:
- 在C. elegans*中进行蛋白质组学和基因操纵.
- 对后代生存率和应激反应表型的分析.
- 化学突变发生屏幕用于识别关键调节基因.
主要成果:
- 母体表达的ETC基因 (*nduf-7*, *isp-1*) 突变等位体改变了后代的奥斯莫斯应激反应,而不依赖于后代的基因型.
- 在卵细胞中表达野生类型的 *isp-1* 恢复了后代的正常应激反应.
- 孕产妇的ETC成分调节后代的AMP激酶功能,在压力期间影响ATP和甘油代谢.
结论:
- 卵细胞ETC成分对于将母体环境暴露与后代的适应性代谢变化联系起来至关重要.
- 孕产妇的ETC成分通过调节AMP激酶活性来影响后代的应激反应,从而调节ATP和甘油代谢.
- 卵细胞线粒体重塑可能是一个进化保守的机制,以优化对母体环境的后代代谢.
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