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Updated: Jun 29, 2025

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer

Published on: June 9, 2018

用于从正在发育的小脑Purkinje细胞中Cre-mediated条件删除floxed等位基的工具

Jennifer N Jahncke¹, Kevin M Wright²

¹Neuroscience Graduate Program, Oregon Health & Science University, Portland, OR 97239, USA.

bioRxiv : the preprint server for biology

|April 8, 2024

概括

评估Cre-lox系统针对小脑Purkinje细胞的评估显示,重组效率和蛋白质损失时间各不相同. Calb1 Cre 线提供精确的胚胎向,而其他线则显示Purkinje 细胞的马赛克或缺乏重组.

科学领域:

神经科学是一个神经科学.
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

在神经科学中,Cre-lox系统对于向基因操纵至关重要.
大脑皮尔金耶细胞对于运动控制和学习至关重要.
准确准Purkinje细胞对于研究它们的功能至关重要.

结论:

不同的Cre-lox工具为准小脑Purkinje细胞提供了多样化的时间和空间控制.
重组后蛋白质损失的验证对于准确的实验解释至关重要.
Calb1 Cre 是一种有前途的工具,可以在Purkinje细胞的早期发育向.

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Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

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相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.