由内源逆转录病毒编码的口腔蛋白是斑马鱼发育所需的
Ni-Chen Chang1, Jonathan N Wells1, Andrew Y Wang1
1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14850, USA.
bioRxiv : the preprint server for biology
|April 8, 2024
概括
斑马鱼中最近活跃的可转移元素 (TE) 编码为胚胎发育必需的蛋白质. 这些活跃的TE,如BHIKHARI-1 Gag,对于中皮和神经发展至关重要.
科学领域:
- 基因组学就是基因组学.
- 发展生物学 发展生物学
- 分子生物学分子生物学
背景情况:
- 可转移元素 (TE) 构成了真核生物基因组的很大一部分.
- 虽然许多TE是不活跃的,但一些古老的TE衍生的序列被重新用于宿主功能.
- 由于研究重复序列的挑战,人们对*活动*TE在有益功能中的作用,特别是发育过程中,了解得很少.
研究的目的:
- 研究最近活跃的可移植元素在胚胎发育中的功能作用.
- 为了确定活性TEs是否编码对生物体发育至关重要的蛋白质.
- 阐明积极的TE可能被整合到发展过程中的机制.
主要方法:
- 使用斑马鱼作为模型生物来研究活性可转移元素.
- 进行了淘汰和救援实验,以评估特定的TE编码蛋白的功能.
- 研究了胚胎中TE衍生的蛋白质的细胞局部化和子宫外表达的影响.
主要成果:
- 证明内源逆转录病毒家族BHIKHARI-1 (Bik-1) 编码了一种对斑马鱼中皮发育至关重要的Gag蛋白.
- 表明Bik-1 Gag与细胞膜结合,并在异位表达时影响细胞迁移.
- 发现BHIKHARI-2 Gag的耗尽导致斑马鱼神经发育缺陷.
结论:
- 斑马鱼中最近活跃的可转移元素编码了对胚胎发育至关重要的功能蛋白质.
- BHIKHARI-1和BHIKHARI-2口腔蛋白分别在中皮和神经的发育中发挥着关键作用.
- 提出了一个"成"模型来解释活跃的TE融入保存的发育途径.
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