对133种生理相关的环境化学物质进行生殖毒性选和表征
bioRxiv : the preprint server for biology
|April 8, 2024
概括
一个高通量C. elegans测定有效选生殖毒素. 该方法确定了13种化学物质,包括四级化合物 (QAC),强调需要进一步评估生殖毒性.
科学领域:
- 毒理学 毒理学 毒理学
- 发育生物学 发展生物学
- 环境健康 环境健康
背景情况:
- 由于复杂性和可扩展性问题,常规动物模型的繁殖毒性评估具有挑战性.
- 环境化学物质暴露对繁殖构成风险,需要有效的测试方法.
研究的目的:
- 适应和验证C. elegans中的高通量体内染色体分离试验,用于生殖毒性查.
- 使用此试验和公共数据源来描述133种化学品的生殖毒性.
- 将体内发现与ToxCast的体内数据进行比较.
主要方法:
- 适应了C. elegans的体内染色体分离试验,用于高通量查.
- 利用ToxCast和ICE的公开数据来选133种化学品.
- 通过独立的体内胚胎活力试验验证实查结果.
- 将C. elegans测定结果与ToxCast的体外数据进行比较.
主要成果:
- 确定了13种生殖毒素,其中四级化合物 (QAC) 具有高度活性.
- 在C. elegans测定中,在顶部和底部化学物质之间没有观察到生物活性或功效 (AC50) 的显著差异.
- 分类化学物质显示出不同的目标类别,对于高Z分数化学物质,类固醇激素目标的过度代表性.
结论:
- 高通量体内C. elegans模型对于可扩展的生殖毒性评估是有价值的.
- 优先评估QAC对生殖的影响至关重要.
- 在体外和体内试验测定之间目标途径的差异需要进一步调查.
更多相关视频
09:49Use of a Battery of Chemical and Ecotoxicological Methods for the Assessment of the Efficacy of Wastewater Treatment Processes to Remove Estrogenic Potency
Published on: September 11, 2016
14.1K
08:00Screening for Endocrine Activity in Water Using Commercially-available In Vitro Transactivation Bioassays
Published on: December 4, 2016
7.5K
相关概念视频
01:20Mate Choice
Mate choice—the decision about whom to mate with—is a type of natural selection, since animals must reproduce to pass down their genes. Mate choice is also called intersexual selection because the behavior occurs between the sexes.
01:27Chemical Agents for Microbial Control
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
01:24iChip
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...