内子-lariat spliceosomes 将 lariats 转换为真正的圆圈:对内子转换的含义
Manuel Ares1,2, Haller Igel1, Sol Katzman1,2
1Center for Molecular Biology of RNA, University of California, Santa Cruz.
bioRxiv : the preprint server for biology
|April 8, 2024
概括
研究人员发现,循环内核RNA是如何使用结合酶体形成的. 这种新的机制,涉及内部-lariat spliceosome (ILS),解释了这些独特的RNA圈在酵母和人类的创建.
科学领域:
- 分子生物学分子生物学
- 在RNA生物学,RNA生物学.
- 基因组学就是基因组学.
背景情况:
- 圆形内核RNAs,不同于 lariats,很少见,它们的形成机制是未知的.
- 之前的研究还没有阐明这些全长圆形RNAs的生物发生.
研究的目的:
- 调查和提出一个新的假设,以形成全长的圆形内核RNAs.
- 确定负责循环内核RNA生物发生的分子机械.
- 为了确定这种机制是否在整个物种中得到保护.
主要方法:
- 使用Saccharomyces cerevisiae作为一个模型生物体.
- 记录来自多个内核的全长和加工的圆形RNA.
- 调查内-拉里亚特结合体 (ILS) 的催化活性.
主要成果:
- 从酵母中的多个内子中确定了全长和新型的加工圆形RNA.
- 有证据表明,内-lariat结合酶体 (ILS) 具有以前未被描述的催化活性.
- 拉里亚特尾巴的3'-OH攻击树枝点,形成一个3'-5'连接的圆圈.
- 类似的圆形RNA是由人类的U2和U12结合体产生的.
- 拼接后的拼接酶体活动可能会在真核生物基因组进化中促进内置转换.
结论:
- 内核-lariat spliceosome (ILS) 具有一种新型的催化活性,负责循环内核RNA生物发生.
- 这种机制在酵母和人体双胞胎细胞中都存在.
- 拼接体的后拼接活动可能通过内置转换在基因组进化中发挥作用.
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