瘤流体的内容调节瘤向细菌的透和有效性
Y Zhan1, B Burkel2, E J Leaman1
1Department of Mechanical Engineering, Virginia Tech, Blacksburg, VA, USA.
bioRxiv : the preprint server for biology
|April 8, 2024
概括
瘤中的原阻碍了基于细菌的癌症治疗 (BBCT) 的透和有效性. 减少原障碍物可能会提高对固体瘤的BBCT疗法的疗效.
科学领域:
- 在瘤学瘤学.
- 微生物学 微生物学
- 生物医学工程 生物医学工程
背景情况:
- 基于细菌的癌症治疗 (BBCT) 利用瘤选择性细菌进行癌症治疗.
- 在固体瘤中有限的细菌殖民是BBCT疗效的关键挑战.
- 已知瘤原蛋白含量会阻碍治疗透,但其对BBCT的影响还未得到充分研究.
研究的目的:
- 为了研究原蛋白含量对瘤内透和抗瘤功能的影响,Salmonella Typhimurium VNP20009 cheY+.
- 为了测试原限制细菌透和随后的抗瘤作用的假设.
主要方法:
- 利用低和高原含量瘤球形模型的三阴性小鼠乳腺癌.
- 使用数学建模和单细胞分辨率分析量化细菌透和分布.
- 通过测量癌细胞死亡来评估抗瘤功效.
主要成果:
- 高原蛋白含量显著降低了细菌的透和分布,大约是7倍.
- 低原蛋白瘤显示~73%的癌细胞死亡增加,而高原蛋白瘤只有28%的增加.
- 数学建模表明,在高原环境中细菌扩散率显著降低,主导了结果.
结论:
- 原蛋白起到物理屏障的作用,阻碍*S. Typhimurium* VNP20009 cheY+的透,并降低了BBCT的疗效.
- 了解和克服原的屏障效应对于设计更有效的BBCT菌株至关重要.
- 准原蛋白或工程细菌来克服它可能会增强殖民和固体瘤的治疗结果.
更多相关视频
08:52Intravital Microscopy of Tumor-associated Vasculature Using Advanced Dorsal Skinfold Window Chambers on Transgenic Fluorescent Mice
Published on: January 19, 2018
13.9K
07:44Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports
Published on: November 28, 2019
7.5K
相关概念视频
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
01:27Tumor Immunotherapy
522
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
522