环保提取:一种绿色方法,最大限度地从南瓜 (Curcubita moschata L.) 中提取生物活性
Milana Matić1,2, Alena Stupar2, Lato Pezo3
1Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
Food chemistry: X
|April 8, 2024
概括
粉的优化加速溶剂提取 (ASE) 有效地回收胡卜素和多. 这种方法增强了抗氧化活性,突出了南瓜的潜在健康益处.
科学领域:
- 食品科学 食品科学 食品科学
- 分析化学 分析化学
- 植物化学 植物化学
背景情况:
- 是一种丰富的生物活性化合物来源,如胡卜素和多.
- 提取方法显著影响这些化合物的产量和特征.
- 了解最佳提取参数对于最大限度地提高生物活性化合物回收至关重要.
研究的目的:
- 为了优化粉中的胡卜素和多的加速溶剂提取 (ASE).
- 评估提取参数对生物活性化合物产量和抗氧化活性的影响.
- 在优化的提取物中识别关键的胡卜素和化合物.
主要方法:
- 使用了加速溶剂提取 (ASE).
- 标准分数 (SS) 和人工神经网络 (ANN) 模型被用于优化.
- 使用二极管阵列检测 (HPLC-DAD) 的高性能液体染色学用于化合物分析.
- 使用DPPH和ABTS试验评估了抗氧化活性.
主要成果:
- 该ANN模型有效地预测了总胡卜素含量 (TCC),总含量 (TPC) 和抗氧化能力.
- 最佳提取条件被确定为50%的溶剂度,120°C,5分钟,2次循环.
- 优化的提取物产生了高TCC (571.49μg/g) 和TPC (7.85 mg GAE/g).
- HPLC-DAD分析证实了主要的胡卜素和化合物的存在.
结论:
- 优化ASE是一种高效的方法,可以从南瓜中提取胡卜素和多.
- 提取的生物活性化合物和抗氧化剂活性之间存在强烈的相关性.
- 这些发现表明,通过优化ASE获得的南瓜提取物与健康相关的显著潜在益处.
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