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IS6110的基因组表征在Mycobacterium orygis中的插入

Ahmed Kabir Refaya¹, Umashankar Vetrivel², Kannan Palaniyandi¹

¹Department of Immunology, ICMR-National Institute for Research in Tuberculosis, Chetpet, Chennai, India.

Evolutionary bioinformatics online

|April 8, 2024

概括

结核菌复合体的亚种Mycobacterium orygis显示出显著的IS6110插入. 这些插入影响宿主适应和遗传多样性,为M. orygis的进化和致病性提供了洞察力.

科学领域:

微生物学微生物学
基因组学就是基因组学.
进化生物学进化生物学

背景情况:

结核菌菌复合体 (MTBC) 菌株的特点是插入元素IS6110,对于遗传修饰和适应性至关重要.
一种MTBC亚种Mycobacterium orygis越来越多地被认为具有包括动物感染潜力在内的One Health影响.
IS6110副本编号与MTBC菌株的进化和适应性有关.

研究的目的:

在M. orygis基因组中全面分析IS6110插入.
为了阐明IS6110插入M. orygis.的遗传后果.
了解IS6110如何影响M. orygis.宿主适应性和病原性.

主要方法:

在67个M. orygis分离物上使用ISMapper对IS6110插入的全基因组分析.
插入位置的识别和表征 (内基因和内基因).
用 eggNOG-mapper 5.0.0 来分析IS6110插入影响的基因的基因本体学和KEGG通路分析.

主要成果:

在分析的M. orygis序列中,91%显示出大量的IS6110插入.
确定了255个不同的IS6110插入位点,其中141个是内基因和114个是内基因.
IS6110插入干扰了63个参与多种基因本体学功能的基因和45个KEGG通路中的基因,影响了代谢过程.

结论:

IS6110转移显著塑造M. orygis基因组,影响其适应性和宿主相互作用.
偏好的插入部位和受影响的基因为M. orygis病原性的遗传基础提供了洞察力.
这项研究增强了对MTBC中M. orygis的进化动态和遗传基础的理解.

关键词:

在IS6110中使用.这种细菌是Mycobacterium orygis.结核菌菌菌菌复合体结核菌菌结核菌复合体一个健康,一个健康.牛结核是牛结核的一种疾病.动物性疾病是动物性疾病.

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