在血管植物出现期间,ACL5获得了严格的热精子合成活性
1Department of Life Science, Faculty of Life Science, Kyushu Sangyo University, 2-3-1 Matsukadai Higashi-ku, Fukuoka, 813-8503, Japan.
The New phytologist
|April 8, 2024
概括
不常见的多氨酸如诺斯胺 (Nspm) 是由植物酶合成的. 这项研究揭示了这些酶如何在植物进化过程中发展出NSPM和热胺 (TSPM) 的基质特异性.
科学领域:
- 植物生物化学 植物生物化学
- 分子进化是分子进化的过程.
- 聚氨酸的新陈代谢 多氨酸的新陈代谢
背景情况:
- 诺斯胺 (Nspm) 是一种不常见的聚胺,在植物和白植物中发现.
- 氨基转移酶 (APT) 是参与聚胺合成的酶.
- 众所周知,ACL5在开花植物中合成热精氨酸 (Tspm).
研究的目的:
- 研究负责NSPM合成的氨基转移酶.
- 确定ACL5基质特异性的进化变化.
- 确定参与基质特异性的ACL5的结构区域.
主要方法:
- 在Nicotiana benthamiana中,APT基因的过渡性高表达.
- 使用气体染色学-质谱学量化聚胺分布的量化.
- 在各种植物物种中对ACL5基因的功能分析.
主要成果:
- 在ACL5的 Bryophyte orthologues 中表现出强烈的 Nspm 合成活性.
- 塞拉吉内拉moellendorffii拥有两个ACL5正态与不同的基质特异性 (Nspm和TSPM).
- 确定ACL5的N端β毛区域对基质特异性至关重要.
结论:
- 在植物进化过程中,ACL5经历了多次基质特异性转变.
- 血管植物的出现与ACL5.5获得严格的TSPM合成活性有关.
- 结构性修改,特别是在β-hairpin区域,驱动了ACL5功能中的这些进化变化.
关键词:
在ACL5中使用ACL5.塞拉吉内拉 (Selaginella moellendorffii) 是一种的植物.氨基基转移酶是一种氨基基转移酶.挪威的精矿是什么意思聚胺聚胺的使用方法基质特异性 基质特异性热精矿是一种热精矿.更多相关视频
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