从Halomonas cupida J9中建立低成本的聚酸基酸生物塑料生产平台
Siqi Wang1, Yujie Liu1, Hongfu Guo1
1Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China.
Biotechnology and bioengineering
|April 8, 2024
概括
工程化Halomonas细菌有效地从廉价,非无菌的原料,如和农业废物中生产聚氨酸酸 (PHA),大大降低了生产成本. 这一突破使得从可持续资源中低成本制造生物塑料成为可能.
科学领域:
- 生物技术是生物技术.
- 合成生物学 合成生物学
- 微生物工程 微生物工程
背景情况:
- 高昂的生产成本限制了聚酸 (PHA) 的制造,主要是由于昂贵的碳来源和绝育要求.
- 开发具有成本效益和可持续的PHA生产方法对于生物塑料的广泛采用至关重要.
研究的目的:
- 通过使用细菌Halomonas cupida J9.建立一个低成本的多基酸盐 (PHA) 生产平台.
- 设计H. cupida J9以高效利用低成本碳源西洛斯,用于PHA生物合成.
- 用农业废物在开放和不无菌条件下证明PHA的生产.
主要方法:
- 开发了一种针对Halomonas cupida J9的无标记基因组编辑系统.
- 改造了H. cupida J9以引入一个氧化糖代谢模块并阻止氧化酸盐的产生,从而产生了J9UΔxylD-P8xylA.菌株.
- 在开放条件下使用葡萄糖/氧化糖联合养和玉米水解盐作为碳来源进行料批发发酵.
主要成果:
- 工程菌株J9UΔxylD-P8xylA实现了迄今为止从Halomonas的西洛中获得的最高PHA产量 (2.81 g/L).
- 证明了第一个从Halomonas的树脂糖中产生短链和中链长度 (SCL-co-MCL) PHA.
- 在5L生物反应器中,在开放,非无菌的条件下,与葡萄糖和西洛斯同时食,达到12.57g/L的PHA.
- 在开放发酵中从玉米水解液中生产2.45g/L的PHA.
结论:
- 改造的H. cupida J9菌株为低成本的PHA生产提供了一个有前途的平台.
- 使用廉价的原料,如西洛斯和农业废物,加上无菌发酵,大大降低了生产成本.
- 这种方法突显了从富含纤维素素农业废物中制造可持续生物塑料的潜力.
相关概念视频
Upstream Processing
102
Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
102
Production of Organic Acids
111
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
111
Production of Biopesticides
120
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
120
Bioplastics
73
Bioplastics derived from microbial processes present a sustainable alternative to conventional petroleum-based plastics. Among these, polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrates (PHBs), have emerged as prominent candidates due to their biodegradability and biocompatibility. These polymers are synthesized by a variety of bacteria, such as Cupriavidus necator and Pseudomonas putida, which naturally accumulate PHAs as intracellular carbon and energy reserves, especially under...
73
Biofuels
108
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...
108
Microbial Bioremediation of Plastics
142
Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
142


