相关实验视频

Updated: Jun 29, 2025

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

An Adoptive Transfer Model of Rheumatoid Arthritis in Mice

Published on: June 6, 2025

通过miR-199a-3p/TXNIP轴缓解IL-1β诱导的骨关节炎

Xi Gu¹, Tian Xie²

¹Department of Orthopedics, Wuhan Hospital of Traditional Chinese Medicine, No. 49 Lihuangpi Road, Jiang'an District, Wuhan, 430014, China.

Biochemical genetics

|April 8, 2024

概括

在骨关节炎 (OA) 中,长非编码RNA AC005165.1 的下调. 它的减少通过miR-199a-3p/TXNIP通路促进了冠状细胞亡和炎症,这表明OA的治疗标.

科学领域:

生物化学生物化学
分子生物学分子生物学
整形外科整形外科整形外科

背景情况:

骨关节炎 (OA) 是一种退行性关节疾病,导致运动障碍和残疾.
长非编码RNAs (lncRNAs) 越来越多地被认为在OA病变发生过程中的关键作用.
了解OA中 lncRNAs的特定机制对于开发向疗法至关重要.

研究的目的:

在骨关节炎的细胞模型中研究 lncRNA AC005165.1 的作用和潜在机制.
为了探索AC005165.1,miR-199a-3p和胆素相互作用蛋白 (TXNIP) 之间的相互作用.

主要方法:

定量实时PCR测量基因表达.
细胞活力测试 (CCK-8) 和亡测试 (流细胞计).
西方涂抹和ELISA用于炎症性细胞因子分析; luciferase reporter和Pearson的相关性测试用于分子相互作用.

主要成果:

在OA患者的软骨和IL-1β治疗的红细胞中,AC005165.1的表达显著下调.
AC005165.1 knockdown 加剧了IL-1β诱导的慢性细胞亡和炎症.
AC005165.1与针对TXNIP的miR-199a-3p直接相互作用,揭示了miR-199a-3p/TXNIP轴.

结论:

AC005165.1通过抑制状细胞亡和炎症,在OA中起着保护作用.
AC005165.1/miR-199a-3p/TXNIP轴是开放式学习发展的一个关键途径.
针对AC005165.1/miR-199a-3p/TXNIP通路可能为骨关节炎提供一种新的治疗策略.

关键词:

在AC005165.1中使用.细胞灭亡 (apoptosis) 是一种死亡的过程.炎症炎症是一种炎症.在TXNIP中,您可以使用TXNIP.在 miR-199a-3p 中.

更多相关视频

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

Software-Assisted Quantitative Measurement of Osteoarthritic Subchondral Bone Thickness

Published on: March 18, 2022

Author Spotlight: Treating Knee Osteoarthritis with Tuina - A New Perspective

Author Spotlight: Treating Knee Osteoarthritis with Tuina - A New Perspective

Published on: January 12, 2024

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...