在Streptomyces中二次代谢物生物合成的转录调节剂
Xinwei Pei1, Yunyun Lei1, Huawei Zhang2
1School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, 310014, China.
World journal of microbiology & biotechnology
|April 8, 2024
概括
本综述总结了Streptomyces中增强二次代谢物 (SM) 生产的转录调节剂. 它探讨了为新型SM生物合成设计这些调节器,推进了代谢工程.
科学领域:
- 微生物学 微生物学
- 代谢工程是代谢工程.
- 合成生物学 合成生物学
背景情况:
- 在Streptomyces代谢工程方面,使用重组DNA技术取得了重大进展.
- 微生物基因组测序和生物信息学揭示了众多的二次代谢物 (SM) 生物合成基因集群及其逻辑.
- 了解转录调节器对于优化Streptomyces中SM生产至关重要.
研究的目的:
- 综合审查特征因素,增强SM生产和激活Streptomyces中的SM生物合成.
- 为了解转录调节器在Streptomyces二次新陈代谢中的作用提供见解.
- 为新的SM发现提供工程转录调节器的未来观点.
主要方法:
- 对Streptomyces中特征转录调节者的文献综述.
- 对SM生物合成基因集群和调控机制的已公布数据的分析.
- 综合有关增强SM生产和生物合成的因素的信息.
主要成果:
- 本文提供了已知的转录调节剂的综合摘要,这些调节剂参与了增强SM产生的过程.
- 审查阐明了这些调节者影响SM生物合成的机制.
- 突出了唤醒沉默或神秘的SM基因集群的关键因素和策略.
结论:
- 转录调节器是增强Streptomyces中二次代谢物产生的关键目标.
- 这些调节器的工程具有发现和生产新型SM的巨大潜力.
- 对转录调节器工程的进一步研究将推动代谢工程和药物发现方面的创新.
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