相关实验视频

Updated: Jun 29, 2025

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
11:04

Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

Published on: September 7, 2019

9.2K

甲诺巴克生物合成的初步步骤:由混合铁酶MbnBC结合基质

Richard J Jodts, Madeline B Ho, Reyvin M Reyes

    Biochemistry
    |April 8, 2024
    PubMed
    概括

    电子核双共振 (ENDOR) 谱学揭示了MbnBC酶复合体的活性Fe (II) Fe (III) 状态. 这项研究详细介绍了基质与混合价值二氧化铁酶 (MVDO) 活性部位的结合,这对甲巴克生物合成至关重要.

    更多相关视频

    Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG12:23

    Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

    Published on: May 16, 2017

    7.5K
    Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues12:07

    Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

    Published on: November 22, 2014

    14.0K

    相关概念视频

    The Electron Transport Chain01:30

    The Electron Transport Chain

    The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
    Inhibitors of the electron transport chain
    Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
    16.7K
    Electron Transport Chain: Complex I and II01:46

    Electron Transport Chain: Complex I and II

    The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
    ROS generation is regulated and maintained at moderate levels necessary...
    13.1K
    Phase II Reactions: Methylation Reactions01:17

    Phase II Reactions: Methylation Reactions

    Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
    The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...
    181
    Conjugate Addition of Enolates: Michael Addition01:08

    Conjugate Addition of Enolates: Michael Addition

    The attack of a nucleophile at the β carbon of an α,β-unsaturated carbonyl compound is called conjugate addition. Conjugate addition reactions of active methylene compounds, such as β-diketones, β-keto esters, β-keto nitriles, and α-nitro ketones, are called Michael addition reactions.
    2.5K
    Phase II Reactions: Miscellaneous Conjugation Reactions01:19

    Phase II Reactions: Miscellaneous Conjugation Reactions

    Phase II biotransformations are detoxification mechanisms that conjugate xenobiotics with endogenous substances, neutralizing their toxicity.
    A key example involves the conjugation of cyanide ions, which impair cellular respiration and alter hemoglobin into non-oxygen-carrying cyanmethemoglobin. To neutralize this threat, a sulfur atom from thiosulphate is transferred to the cyanide ion, catalyzed by the enzyme rhodanese, resulting in an inactive compound called thiocyanate. The production of...
    59