微塑料和纳米塑料对人类肠道有机体衍生的表皮质的影响
Brooke Wang1, Lilianne Iglesias-Ledon1, Matthew Bishop1
1Department of Biomedical Engineering, Tufts University, Medford, Massachusetts.
Current protocols
|April 8, 2024
概括
本研究提出了一种简化方案,用于创建人类肠道有机单层,包括微细胞,以调查微塑料和纳米塑料对肠道健康和屏障功能的影响.
科学领域:
- 胃肠病学和肝病学
- 毒理学和环境健康
- 干细胞生物学和再生医学
背景情况:
- 与传统细胞系相比,来自患者的肠道器官提供了人类肠道上皮质的优越体外模型.
- 了解微塑料 (MP) 和纳米塑料 (NP) 等环境污染物对肠道的吸收和生物影响至关重要.
研究的目的:
- 详细介绍一项简化方案,用于生成由人类肠道有机体衍生的单层,带有或没有微细胞 (M细胞).
- 提供验证的方法来表征MP/NP,并评估它们的吸收和对肠道上皮细胞的影响.
- 为了研究塑料颗粒吸收机制和对人类肠道的生物影响.
主要方法:
- 人类肠道有机体的培养和分化成单层.
- 使用扫描电子显微镜 (SEM) 和共聚焦免疫染用于吸收研究的MP/NP的表征.
- 通过细胞因子分析评估肠道屏障功能,使用超内皮电阻 (TEER) 和通过细胞因子分析评估炎症反应.
主要成果:
- 成功生成由有机体衍生的单层,可选地包括M细胞,以促进体外粒子吸收的研究.
- 用于可视化MP/NP吸收并量化其对肠道屏障完整性和炎症标志物的影响的验证协议.
- 在研究MPs/NPs对人肠表皮的作用时,有机体模型的实用性已被证明.
结论:
- 开发的协议为研究塑料颗粒对人类肠道健康的影响提供了一个强大的系统.
- 该模型允许在生理学上相关的背景下调查颗粒吸收和炎症反应.
- 这种方法适用于研究其他环境暴露及其对肠道生理学的影响.
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