角膜炎症细胞的形态差异化
Fynn Schmitz1,2, Rafael Klimas1,2, Marie Spenner1,2
1Department of Neurology, St. Josef-Hospital, Ruhr-University Bochum, Bochum, Germany.
Cornea
|April 8, 2024
概括
使用共聚焦显微镜进行角膜炎症细胞 (CIC) 分类的标准化提高了诊断可靠性. 一个新的协议增强了分类器之间的协议,使得CICs能够进行疾病活动评估的一致评估.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 医疗成像医学成像
背景情况:
- 角膜共聚焦显微镜 (CCM) 分析角膜神经纤维和角膜炎症细胞 (CIC).
- CICs是慢性自身炎症性疾病中疾病活性的潜在生物标志物.
- 目前缺乏标准化的标准,阻碍了可靠的CIC形态表征.
研究的目的:
- 建立一个标准化的协议,用于CIC的形态分类.
- 评估协议的适用性和评价者之间的可靠性.
主要方法:
- 系统的文献审查以确定CIC定义.
- 结构化分类算法的开发和代改进.
- 通过多个评级者应用优化协议,并使用Fleiss kappa计算评级者之间的可靠性.
主要成果:
- 在文献搜索中发现,对于区分CIC类型没有统一的标准.
- 最初的协议显示了较低的分级间协议 (K = 0.09).
- 修订后的协议实现了显著更高的评级者间可靠性 (K = 0.64与3评级者,K = 0.61与4评级者).
结论:
- 目前基于文献的CIC标准导致错误率很高.
- 一个具有明确定义的细胞类型的标准化协议可以产生可重复的结果.
- 开发的协议适用于未来对角膜共聚焦显微镜中CIC的评估.
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