在先天性隔膜的人类和小鼠模型中,内皮细胞转化为半膜细胞的转变
Jamie Gilley1,2, Sandra K Hanneman1, Madelene J Ottosen1
1UTHealth Houston Cizik School of Nursing, Houston, Texas, USA.
Neonatology
|April 8, 2024
概括
内皮转介质过渡 (EndoMT) 可能会在先天性隔膜 (CDH-PH) 中导致肺高血压. 在CDH细胞中的α-SMA增加表明EndoMT在CDH-PH血管改造中发挥作用.
科学领域:
- 新生儿研究新生儿研究
- 血管生物学 血管生物学
- 遗传性疾病 遗传性疾病
背景情况:
- kongenital diaphragmatic hernia (CDH) 导致肺高血压 (PH),新生儿的死亡率高.
- 肺血管细胞外矩阵 (ECM) 改造,可能是通过内皮转移到介质细胞转换 (EndoMT),涉及到CDH-PH.
- 关于EndoMT在CDH-PH病变发生中的特定作用的数据有限.
研究的目的:
- 调查EndoMT对CDH-PH的贡献.
- 通过评估α-SMA表达来识别接受EndoMT的细胞.
- 分析人类静脉内皮细胞 (HUVEC) 和小鼠CDH模型中的EndoMT标记物.
主要方法:
- 来自CDH和对照组的人类脉内皮细胞 (HUVEC) 暴露于TGF-β.
- 用尼特罗诱导的CDH模型研究了小鼠幼.
- 进行了α-SMA和CD31的免疫组织化学,并使用斐济软件量化表达.
主要成果:
- 在CDH HUVEC中,α-SMA表达显著增加了1.1倍 (p=0.02).
- 在小鼠模型中,托芬诱导的CDH幼与对照动物相比呈现了增加α-SMA (0.4倍) 和降低CD31表达 (0.8倍) 的趋势,尽管在统计学上并不显著.
结论:
- 在CDH-PH中观察到的肺血管重塑中,内皮细胞转移到介质细胞转换 (EndoMT) 可能起作用.
- 这些发现表明EndoMT是CDH-PH的潜在治疗标.
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