工程设计含有白醇的奇托桑纳米颗粒,用于对抗Helicobacter pylori感染的潜在用途
Larissa Spósito1, Diana Fonseca2, Suzana Gonçalves Carvalho3
1São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Drugs and Medicines, Araraquara, SP, Brazil; São Paulo State University (UNESP), School of Pharmaceutical Sciences, Department of Biological Sciences, Araraquara, SP, Brazil; i3S-Instituto de Investigação e Inovação em Saúde, Rua Alfredo Allen, 208, Porto 4200-135, Portugal; INEB-Instituto de Engenharia Biomédica, Rua Alfredo Allen, 208, Porto 4200-135, Portugal.
装有转白 (RESV-NP) 的奇托桑纳米颗粒对Helicobacter pylori表现出强烈的活性,有效地消灭浮游生物细菌和生物膜. 这种新的配方为管理H. pylori感染提供了一个有前途的战略,具有低毒性.
科学领域:
- 纳米技术 纳米技术
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 杆菌感染与严重的胃病有关.
- 由于毒性因素和生物膜形成,H. pylori表现出抗生素耐药性,需要新的治疗策略.
- 转白醇 (RESV),一种天然的多,显示出对H.pylori的潜在活性.
研究的目的:
- 开发和表征含有转白醇 (RESV-NP) 的奇托桑纳米颗粒,用于H. pylori的管理.
- 评估 RESV-NP 对 H. pylori 在浮游生物和生物膜形式以及体外感染模型中的疗效.
- 评估RESV-NP的细胞毒性和急性毒性.
主要方法:
- 通过离子凝制备RESV-NP,并使用DLS,NTA和Cryo-TEM进行表征.
- 用HPLC确定封装效率和体外释放.
- 进行了抗微生物活性 (MIC/MBC,杀时),生物膜根除,体外感染模型疗效和细胞毒性测试.
主要成果:
- RESV-NP表现出球形形态,尺寸为~145nm,具有正的泽塔潜力.
- 观察到高封装效率 (72%) 和快速释放 (<5分钟) 的RESV.
- RESV-NP表现出强大的抗菌活性 (MIC/MBC:3.9μg/mL),在2xMIC消除了H. pylori生物膜,并在体外感染模型 (4xMIC) 中显著降低了细菌负载.
- 在MIC度下对AGS和MKN-74细胞系没有观察到细胞毒性,在Galleria mellonella中也没有发现急性毒性.
结论:
- RESV-NP代表了H. pylori管理的有效策略,显示出显著的抗微生物和抗生物膜活性.
- RESV-NP的粘膜粘合性表明,它有可能用于口服,用于治疗与H. pylori相关的胃疾病.
- 这种纳米粒子配方为H. pylori感染治疗提供了一个有希望的低毒性替代品.
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