相关实验视频

Updated: Jun 29, 2025

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

第三类干扰素通过重编程巨细胞介导的细胞分裂和编排有效的免疫反应来抑制膀癌的进展

Bo Wang^1,2, Bingkun Zhou^1,2, Junyu Chen^1,2

¹Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen (Zhongshan) University, Guangzhou, China.

Journal for immunotherapy of cancer

|April 8, 2024

概括

第三种类型的干扰素 (IFN-λ3) 通过促进免疫细胞透和巨细胞化在膀癌中来增强抗瘤免疫力. 这表明IFN-λ3作为一种潜在的生物标志物和治疗点,可以改善免疫治疗的结果.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
分子生物学分子生物学

背景情况:

干扰素 (IFN) 对免疫反应和癌症免疫疗法至关重要.
第三类干扰素 (IFN-λ) 在各种疾病中起作用,但其对瘤免疫微环境的影响尚不清楚.
研究IFN-λ的直接作用对于开发新型癌症疗法至关重要.

研究的目的:

研究III型干扰素-λ3 (IFN-λ3) 对瘤免疫微环境的直接影响.
评估IFN-λ3作为预测性生物标志物和膀癌的免疫治疗剂的潜力.

主要方法:

使用小鼠MB49膀瘤模型与逆转录病毒载体介导的IFN-λ3表达.
在免疫能力高和T细胞缺乏的瘤环境中评估了抗瘤作用.
分析了人类膀癌样本 (n=35) 使用免疫组织化学和多重免疫光学来将IFN-λ3表达与免疫细胞透和治疗反应相关联.

主要成果:

在瘤中异位IFN-λ3表达增加了细胞毒性CD8+T细胞,Th1细胞,NK细胞和促炎性巨细胞,同时减少了中性粒细胞.
转录组分析揭示了免疫反应基因的升高调节,增强了淋巴细胞的招募,激活和细胞化.
人类膀癌中的IFN-λ3表达与效应器免疫透物和免疫检查点阻塞疗法的疗效正相关.

结论:

IFN-λ3促进了巨细胞介导的细胞分裂,并增强了抗瘤免疫反应.
IFN-λ3表达作为在膀癌中免疫疗法疗效的预测生物标志物.
第三种类型的干扰素为膀癌治疗提供了一个有希望的治疗候选者.

关键词:

免疫检查点抑制剂免疫检查点抑制剂巨细胞是一个巨细胞.发细胞症 (phagocytosis) 是一种致死细胞的发生.尿囊新生体是什么意思

更多相关视频

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...