酵母TLDc域蛋白调节V-ATPase的组装状态和亚细胞局部化
Samira Klössel1, Ying Zhu2, Lucia Amado1
1Osnabrück University, Department of Biology/Chemistry, Cellular Communication Laboratory, Barbarastrasse 13, 49076, Osnabrück, Germany.
The EMBO journal
|April 8, 2024
概括
两个酵母蛋白,Rtc5和Oxr1,调节真空型质子 (V-ATPase) 的分解和功能. 这些TLDc-域蛋白质影响V-ATPase组合和亚单元定位,揭示了真空调节的新见解.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 酵母真空体是具有多种功能的必需器官,包括降解,储存和信号传递.
- 真空类型的H+-ATPase (V-ATPase) 是一个关键的蛋白质复合体,负责真空酸化.
- 了解V-ATPase调节对于理解真空子的功能至关重要.
研究的目的:
- 为了确定酵母中V-ATPase的新型蛋白相互作用和调节剂.
- 研究TLDc域蛋白质,特别是Rtc5和Oxr1在V-ATPase功能中的作用.
- 阐明Rtc5和Oxr1影响V-ATPase组装,拆卸和定位的机制.
主要方法:
- 在分离的酵母真空中进行交叉连接质谱,以确定蛋白质与蛋白质之间的相互作用.
- 使用酵母遗传学对Rtc5和Oxr1功能的体内分析.
- 评估对Rtc5和Oxr1.1的反应中V-ATPase组装,拆卸和子单位定位的评估.
主要成果:
- 确定了Rtc5作为V-ATPase的一个新型交互因子.
- 证明Rtc5和Oxr1都在体内促进V-ATPase分解.
- 表明Rtc5和Oxr1抵消了V-ATPase组合的伴侣复合体,RAVE.
- 发现Oxr1对于在戈尔吉器官中保留戈尔吉特异性V-ATPase亚单元至关重要.
结论:
- 酵母TLDc域蛋白Rtc5和Oxr1是V-ATPase功能的新型调节者.
- 这些蛋白质在V-ATPase动态中起着相反的作用,影响分解和组装.
- Oxr1在V-ATPase亚单元局部化到高尔基细胞中发挥着特定的作用.
- 这些发现突显了体内V-ATPase的复杂,多方面的调节.
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