大气压场吸附离子移动性-质谱学合
1Institute of Organic Chemistry, Heidelberg University, Im Neuenheimer Feld 270, 69120, Heidelberg, Germany. juergen.gross@oci.uni-heidelberg.de.
Analytical and bioanalytical chemistry
|April 8, 2024
概括
大气压场吸附离子移动性质谱学 (APFD-TIMS-MS) 允许对极性化合物和多环芳进行分析. 这种新技术扩大了分析剂的范围,并改善了各种分子的检测极限.
科学领域:
- 分析化学 分析化学
- 质谱测量质量谱测量
- 分离科学 分离科学
背景情况:
- 已建立的软真空电离方法,如场电离 (FI) 和场脱离 (FD),在质谱学 (MS) 中至关重要.
- 大气压场脱吸 (APFD) 是一种最近开发的技术,提供与FI和FD相似的电离能力.
- APFD可以产生极性/离子化合物的正偶电子离子和多环芳香化合物的分子离子 (M+•).
研究的目的:
- 构建和演示一个专用的大气压场脱吸 (APFD) 源组件,以实现强大的运行.
- 在四极飞行时间 (Q-TOF) 仪器上探索APFD与被困离子移动性光谱学 (TIMS) 的联合使用.
- 描述新型大气压场吸附离子移动性-质谱学 (APFD-TIMS-MS) 合的操作特性和能力.
主要方法:
- 开发一个专用的APFD源组件,具有发射器观测和电阻加热功能.
- 集成APFD源与被困离子流动性-四极-飞行时间 (TIMS-Q-TOF) 质谱仪.
- 应用APFD-TIMS-MS用于分离寡合物和确定碰撞横截面 (CCS).
主要成果:
- 该APFD源表现出强大的运行,并通过电阻发射器加热扩大了可访问分析物的范围.
- APFD-TIMS-MS成功地分离了聚乙烯甘醇和氨基终结聚乙烯甘醇的寡合体,分别形成[M+Na]+和[M+H]+离子.
- 对[60]富勒烯和多环芳 (PAHs) 的分析产生了具有低检测极限的分子离子 (M+•) 和分子离子 (M-•) ([a]pyrene的LODs <1 pg).
结论:
- 开发的APFD源具有多功能性,可以适应各种具有大气压接口的布鲁克质谱仪.
- APFD-TIMS-MS 合提供了有效的寡合体分离和准确的CCS测定.
- 该技术显示出分析各种化合物的潜力,包括极性分子,PAH和富勒伦,具有高灵敏度.
关键词:
活动场发射器的发射器.环境脱吸/离子化 (ADI)大气压场绝吸法 (APFD) 是指大气压场绝吸法.发射器的加热电流 (EHC)场绝吸 (FD) 是指场绝吸.电场电离 (FI) 是指电场的电离.捕获的离子移动性光谱法 (TIMS)捕获的离子移动性-四极-飞行时间 (TIMS-Q-TOF)更多相关视频
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