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Updated: Jun 29, 2025

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尿液中的提米丁二分离体排泄反映了个人紫外线辐射暴露水平

Catharina Margrethe Lerche^1,2, Nynne Johanne Sahl Frederiksen³, Ida Schwarz Thorsteinsson³

¹Department of Dermatology, Copenhagen University Hospital-Bispebjerg and Frederiksberg, 2400, Copenhagen, Denmark. catharina.margrethe.lerche@regionh.dk.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology

|April 8, 2024

概括

尿液中的提米丁二聚体水平准确地反映了紫外线 (UVR) 暴露导致的DNA损伤. 这种新方法量化尿液中的二聚体,将其与个人紫外线暴露相关,有助于DNA损伤评估.

科学领域:

生物化学生物化学
皮肤病学皮肤病学
环境健康环境健康

背景情况:

紫外线辐射 (UVR) 暴露会导致皮肤DNA受损,主要是通过循环butan胺二元体,其中胺二元体是最常见的.
在尿液中定量提米丁二聚体作为生物标志物用于UVR诱导的DNA损伤的程度.

研究的目的:

开发和验证一种液态染色体质谱法 (LC-MS) 方法,用于量化尿胺二次数.
在现实生活环境中评估个人紫外线暴露和尿道胺二聚物分泌之间的相关性.

主要方法:

一组27名健康的丹麦志愿者在冬季假期期间佩戴个人紫外线剂量计,随着紫外线暴露的增加.
收集了尿液样本,并使用LC-MS测量了蒂米丁二元度.
个人UVR暴露量以标准红斑剂量 (SEDs) 量化.

主要成果:

在增加紫外线辐射暴露后的1天和2天,尿液中的蒂米丁二元度显著升高.
在48小时尿道胺二聚物分泌和红斑权重个人紫外线暴露之间发现了强烈的相关性 (r2 = 0.64,p < 0.001).
性别,年龄和皮肤类型并没有显著影响紫外线暴露与蒂米丁二元分泌之间的相关性.

结论:

通过LC-MS测量尿液中的提米丁二元分泌量,是个人紫外线照射暂时增加的敏感指标.
该方法提供了一种可靠的方法来量化DNA损伤和修复,并评估光保护策略的有效性.

关键词:

循环butan 胺二聚体是一种二聚体.在 LCMS 中使用.个人剂量计个人剂量计提米丁二聚物是二聚物.氨酸二次体是什么意思紫外线辐射的紫外线辐射.尿液分析尿液分析

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