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温和的γ-丁烯/水预处理,从玉米炉中高效地生产糖

Yu Yang¹, Xueliang Ma¹, Manzhu Wang¹

¹College of Forestry, Northwest A&F University, No.3 Taicheng Road, Yangling, Shaanxi, 712100, China.

Applied biochemistry and biotechnology

|April 8, 2024

概括

γ-丁烯/水 (GBL/H2O) 预处理有效地增强了玉米炉 (CS) 的水解. 这种具有成本效益的方法显著增加了糖的释放,为生物质转化提供了可持续的解决方案.

科学领域:

生物质预处理生物质预处理
绿色化学绿色化学
生物化学工程生物化学工程

背景情况:

玉米炉 (CS) 是一个丰富的纤维素生物质资源.
有效的预处理对于释放CS在生物燃料和生物化学生产方面的潜力至关重要.
目前的预处理方法经常面临效率,成本或环境影响方面的挑战.

研究的目的:

研究g-butyrolactone/水 (GBL/H2O) 作为玉米炉的温和,高效和成本效益的二元溶剂预处理.
优化GBL/H2O预处理参数 (时间,温度,H2SO4度) 以提高水解性.
评估双酶策略的有效性,以改善预处理后释放糖的效果.

主要方法:

系统地调查GBL/H2O预处理参数对玉米炉的物理化学特性.
在不同的条件下分析纤维素结晶性和素去除.
实施双酶水解方案,使用细胞酶补充β-葡萄糖酶或西兰酶.
葡萄糖和西兰的转换率的量化.

主要成果:

在GBL/H2O预处理中,温度升高和H2SO4度显著降低了纤维素结晶性,并促进了素的去除.
双酶策略提高了水解效率,特别是在不同的条件下预处理的CS.
最佳的预处理 (150mM H2SO4,120°C,1小时) 实现了高葡萄糖 (98%) 和西兰 (82.3%) 的转化.
与其他二进制系统相比,GBL/H2O预处理显示出更高的性能,在较低的酶负载下获得更高的糖产.

结论:

GBL/H2O二元溶剂预处理是一种高效和经济的方法,可以提高玉米炉的水解能力.
优化的预处理条件和双酶方法显著提高了生物质转化效率.
本研究提出了可持续生物质利用的有希望的战略,与绿色经济原则保持一致.

关键词:

辅助酶辅助酶是一种辅助酶.二元溶剂系统二元溶剂系统酶性水解是一种酶性水解.GBL/H2O 预处理

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