通过抗CD22 CAR-NK细胞疗法杀死淋巴瘤细胞的高产量杀死
Mahnoosh Abbaszade Dibavar1, Masoud Soleimani2,3, Mohammad Hossein Mohammadi4
1Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
In vitro cellular & developmental biology. Animal
|April 8, 2024
概括
针对CD22的化学抗原受体NK-92 (CAR-NK-92) 细胞对B细胞淋巴瘤治疗有希望. 这些工程细胞有效地杀死淋巴瘤细胞并释放有益的细胞因子,提供一种潜在的更安全的免疫疗法替代方案.
科学领域:
- 免疫学 免疫学 免疫学
- 在瘤学瘤学.
- 生物技术是生物技术.
背景情况:
- 化学抗原受体 (CARs) 为B细胞恶性瘤提供了新的基于免疫的向.
- 汽车T细胞疗法是有效的,但复杂的生产.
- 由于寿命缩短和细胞因子毒性降低,CAR-NK细胞提供了更安全的替代方案,NK-92细胞是CAR工程的可行来源.
研究的目的:
- 研究CD22作为B细胞淋巴瘤中CAR-NK-92治疗的治疗标.
- 为了生成和评估m971-CD28-CD3ζ NK-92细胞的疗效,这些NK-92细胞被设计为向CD22.
主要方法:
- 使用lentiviral转导生成了m971-CD28-CD3ζ NK-92细胞,这些细胞向CD22.
- 流细胞计评估了CAR表达和7-AAD试验,确定了对CD22阳性拉吉细胞的细胞毒性.
- 通过ELISA测量了与细胞共培后的细胞因子产量 (IFN-γ,GM-CSF,IL-6).
主要成果:
- 成功生成了表达CD22特异性CAR的m971-CD28-CD3ζ NK-92细胞.
- CAR-NK-92细胞表现出CD22表达性淋巴瘤细胞系的高效溶解.
- 在与细胞共同培养后,观察到IFN-γ和GM-CSF的显著产生,IL-6水平较低.
结论:
- m971-CD28-CD3ζ NK-92细胞疗法是治疗B细胞淋巴瘤的一个有希望的方法.
- 用CAR-NK-92细胞向CD22提供了一个潜在的更安全和更有效的免疫疗法策略.
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