基化EDTA增强了protoporphyrin的抗菌光动力学活性
Ying Piao1, Sebastian Himbert2, Zifan Li1
1Zhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Journal of nanobiotechnology
|April 8, 2024
概括
一种新的光动力学疗法使用原波林IX (PpIX) 和C14-EDTA显示强大的抗菌作用对抗抗生素耐药的金黄色葡萄球菌和生物膜,提供了一个有希望的替代传统抗生素.
科学领域:
- 生物化学 生物化学
- 摄影化学的使用.
- 微生物学 微生物学
背景情况:
- 由于缺乏新药开发,抗生素耐药性是一个日益严重的全球健康问题.
- 基于Protoporphyrin IX (PpIX) 的光动力学疗法 (PDT) 是一种潜在的替代抗菌策略.
- 较差的PpIX溶解性和聚合性限制了其光动力学功效.
研究的目的:
- 调查化EDTA衍生物 (aEDTA),特别是C14-EDTA的潜力,以提高PpIX的溶解性和抗菌光动力学活性.
- 评估C14-EDTA/PpIX组合对金黄色葡萄球菌 (S. aureus) 和其生物膜的疗效.
- 阐明增强抗菌作用背后的机制.
主要方法:
- 合成和C14-EDTA的表征.
- 使用和不使用C14-EDTA的PpIX的可溶性研究.
- 在LED照射下对S. aureus浮游生物细胞和生物膜进行体外抗菌检测.
- 在感染的小鼠模型中进行体内疗效测试.
- 机理学研究包括膜透性测定和双价离子化分析.
主要成果:
- C14-EDTA显著提高了PpIX在水溶液中的溶解性和分散性.
- 这种C14-EDTA/PpIX组合显示出强大的抗菌活性,可对抗S. aureus,并有效根除S. aureus生物膜.
- 该疗法在感染的动物模型中显示出高疗效.
- 机理学研究表明,C14-EDTA破坏了PpIX结晶,增加了细菌膜的透性,并扣留双价离子,从而改善了PpIX的吸收和增强了反应性氧物种 (ROS) 的产生.
结论:
- C14-EDTA有效地克服了抗菌PDT的PpIX溶解度和聚合的局限性.
- C14-EDTA/PpIX组合代表了一种有前途的策略,用于对抗抗生素耐药的黄金色杆菌感染,包括生物膜.
- 这种方法为治疗具有挑战性的细菌感染提供了传统抗生素的可行替代方案.
相关概念视频
01:19Masking and Demasking Agents
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EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
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01:18Effects of EDTA on End-Point Detection Methods
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Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
272
01:26EDTA: Auxiliary Complexing Reagents
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EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
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01:22EDTA: Chemistry and Properties
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Polydentate ligands are most widely used in complexometric titrations because they form more stable complexes with the metal ions than mono- or bidentate ligands due to the chelate effect. Examples of polydentate ligands are ethylenediaminetetraacetic acid (EDTA), crown ethers, and cryptands. The most important feature of optimal polydentate ligands is the ability to form 1:1 complexes in a single-step process. Amino carboxylic acid derivatives are frequently used as complexing agents. EDTA is...
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01:30EDTA: Direct, Back-, and Displacement Titration
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The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
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01:23EDTA: Indirect and Alkalimetric Titration
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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
909