大米转录因子OsWRKY37在铜缺乏的情况下积极调节开花时间和谷物肥力
Chenchen Ji1,2, Haixing Li3, Jingli Ding1,2
1Research Center of Microelement, Huazhong Agricultural University, Wuhan 430070, China.
Plant physiology
|April 9, 2024
概括
OsWRKY37调节了大米中铜 (Cu) 的吸收和运输,这对花期和产量至关重要. 这种WRKY转录因子增强了缺陷下的Cu分布,改善了谷物生产.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 铜 (Cu) 对于大米 (Oryza sativa) 的开花和产量至关重要,但其吸收和分配的监管机制尚不清楚.
- 有效的Cu转移到子中对于大米的繁殖成功至关重要.
研究的目的:
- 识别和描述涉及水吸收,转移和分布的调节因素.
- 调查 WRKY 转录因子 OsWRKY37 在 Cu 稳态中的作用及其对大米产量的影响.
主要方法:
- 对OsWRKY37基因的分离和表征.
- 在不同的Cu条件下进行基因表达分析 (RT-qPCR,现场RT-PCR).
- 使用过度表达和突变线 (例如,oscopt6) 的功能分析.
- 分子相互作用研究 (酵母单杂交,EMSA,短暂的共同表达).
主要成果:
- OsWRKY37的表达是由Cu缺乏引起的,并且局部存在关键的水组织中.
- 过度表达OsWRKY37增强了Cu缺乏条件下的Cu吸收和转移.
- OsWRKY37直接结合并激活Cu转运基因OsCOPT6和OsYSL16.6的促进者.
- OsWRKY37的突变导致Cu水平降低,花粉发育受损,花期延迟,并在Cu缺乏下降谷物产量.
结论:
- 米中的OsWRKY37作为Cu吸收,根到芽转移和分布的积极调节者.
- 在Cu缺乏条件下,OsWRKY37对OsCOPT6和OsYSL16的激活对花粉发育,开花,生育和谷物产量至关重要.
- OsWRKY37 是一种潜在的基因标,可以在Cu-限制的环境中提高大米产量.
更多相关视频
相关概念视频
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Biological Clocks and Seasonal Responses
34.6K
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
34.6K
Transcription
146.9K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
146.9K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Regulation of Expression at Multiple Steps
902
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
902
Transcription Attenuation in Prokaryotes
15.3K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.3K


