通过双酶固定化方法提高素和脂酶的催化性能
1Department of Chemistry, Faculty of Science, Gazi University 06500 Ankara Turkey nursens@gazi.edu.tr nrnsari@gmail.com.
RSC advances
|April 9, 2024
概括
与铁素结合的奇托桑聚合物有效地固定了素和脂酶酶. 这种双重酶固定增强了酶活性和稳定性,显着增加了Vmax和基质亲和力.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 酶固定化 酶固定化
背景情况:
- 奇托是一种多功能生物聚合物,具有酶不动化的潜力.
- 铁素合增强了聚合物的特性,用于生物分子的附着.
- 开发高效的双酶固定系统对于生物催化剂至关重要.
研究的目的:
- 合成和特征化铁-合基 (CS-Fc) 作为酶固定化的支持.
- 研究单一和双重酶系统 (素和脂酶) 的固定.
- 评估双酶固定对酶活性,稳定性和运动参数的影响.
主要方法:
- 合成铁-合奇托 (CS-Fc) 聚合物.
- 使用FTIR光谱和泽塔电位测量进行表征.
- 单独和作为双酶系统的素和脂酶的固定.
- 评估酶活性,最佳条件,Vmax,基质亲和力和可重复使用性.
主要成果:
- FTIR光谱证实了素和脂酶与CS-Fc聚合物的结合.
- 双酶固定维持了最佳pH值,并将最佳温度提高了10°C.
- 素的Vmax增加了1.75倍,脂酶的3.94倍;基质亲和度增加了0.4倍 (素) 和0.3倍 (脂酶).
- 在5次重复使用后,素的酶保留率为18.21%,脂酶为90.81%.
结论:
- 与铁素合的奇托是有效的双酶固定化的合适支持.
- 支持CS-Fc增强了固定素和脂酶的催化效率和稳定性.
- 这项研究表明了开发强大的生物催化酶系统的有希望的方法.
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