格里克酸化并激活KIN10,这也促进了其降解
Jing Sun1, Hui Liu1, Jantana K Blanford1
1Biology Department, Brookhaven National Laboratory, Upton, NY, United States.
Frontiers in plant science
|April 9, 2024
概括
双胞胎病毒代表相互作用激酶 (GRIK1/2) 调节糖非发酵-1-相关激酶1 (SnRK1) 的稳定性. GRIK1可化并降解KIN10,控制能量恒温.
科学领域:
- 植物分子生物学 植物分子生物学
- 代谢调节 代谢调节 代谢调节
- 信号传导是指信号的传导方式.
背景情况:
- 糖不发酵-1-相关激酶1 (SnRK1) 对于能量恒温至关重要.
- KIN10是SnRK1的关键催化子单元,受到复杂的调节.
- 双子病毒代理相互作用基因酶 (GRIK1和2) 是KIN10.0的上游调节者.
研究的目的:
- 研究GRIK1和GRIK2在KIN10调节中的作用.
- 为了阐明由GRIKs介导的KIN10降解的机制.
- 了解GRIK介导的KIN10调节对代谢恒温的影响.
主要方法:
- 在 *Nicotiana benthamiana* 叶子中进行过渡性蛋白质表达测试.
- 在 *grik1-1 grik2-1* 双重突变植物中分析KIN10蛋白水平.
- 对KIN10 T-循环的酸化位点分析.
主要成果:
- GRIK1酸化KIN10并启动其降解.
- 通过GRIK介导的KIN10降解需要GRIK激酶活性和KIN10T循环酸化.
- 包括一个较长的异型 (KIN10L) 在内的KIN10蛋白质在*grik1-1 grik2-1*双突变中得到了丰富.
- 在GRIK激活时,KIN10稳定性的降低允许在能量水平恢复时,KIN10活动的快速降低.
结论:
- 格里克酶作为KIN10稳定的负调节剂,提供了快速代谢调整的机制.
- 在低糖条件下,GRIK-KIN10相互作用对于防止代谢过度反应至关重要.
- 在没有GRIK活动的情况下,导致KIN10L的替代拼接增强,这表明在长时间的低能信号传输中发挥了作用.
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