马拉塞斯结节上皮和上皮残留之间的发育关系
Shubo Li1,2, Shufang Li2, Mingguo Cao1
1Department of Stomatology, College of Medicine and Health, Lishui University, Lishui, Zhejiang Province, China.
The International journal of developmental biology
|April 9, 2024
概括
在发育中的老鼠牙中,素17 (K17) 表达在结节上皮 (JE) 和马拉塞斯上皮残留物 (ERM) 之间有所不同. 这项研究澄清了它们独特的免疫类型,表明K17不受Lef-1的调节.
科学领域:
- 口腔生物学 口腔生物学
- 发展生物学 发展生物学
- 皮质生物学 皮质生物学
背景情况:
- 氨酸17 (K17) 是淋巴细胞增强因子-1 (Lef-1) 的潜在目标基因.
- K17区分了结点上皮质 (JE) 和马拉塞斯上皮质残留 (ERM),但它们的关系和Lef-1的作用尚不清楚.
- 在JE和ERM中,各种质素 (K5,K6,K7,K14,K16) 的表达模式基本上没有报告.
研究的目的:
- 在产后牙发育过程中研究质素K5,K6,K7,K14,K16,K17和Lef-1的表达.
- 为了澄清与氨酸表达有关的JE和ERM的免疫类型.
- 确定K17表达是否与这些组织中的Lef-1相关.
主要方法:
- 免疫组织化学测试对Wistar大鼠的上进行了测试,从出生后的3.5日到21日.
- 在正在发育的牙组织中分析了K5,K6,K7,K14,K16,K17和Lef-1的表达水平.
- 特别关注结节上皮质 (JE) 和马拉塞斯上皮质残留物 (ERM).
主要成果:
- 在内上皮质 (IEE),减少的上皮质 (REE),ERM和JE中,K5和K14的表达很高.
- K17最初以IEE表达,变为负值,然后通过PN16-21强烈地表达在REE和ERM中,但在JE中仍然负值.
- K6,K7,K16和Lef-1在研究的组织中没有显著的表达 (K16的口腔上皮除外).
结论:
- 减少的面上皮质 (REE) 和马拉塞斯的上皮残留物 (ERM) 在牙喷发之前具有相同的角质形状.
- 交界上皮 (JE) 呈现出与ERM不同的质形状,特别是在爆发后的K17表达中.
- 氨酸17 (K17) 表达与Lef-1无关,这表明JE免疫类型的独特调节途径.
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