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Updated: Jun 29, 2025

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河马ACE2与SARS-CoV-2结合的分子基础

Ruirui Yang^1,2, Pu Han², Pengcheng Han³

¹College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong, China.

Journal of virology

|April 9, 2024

概括

河马ACE2 (hiACE2) 有效地结合SARS-CoV-2尖端蛋白,包括变种. 独特的hiACE2残留物影响结合,表明河马和鱼中潜在的SARS-CoV-2感染风险,需要监测.

科学领域:

病毒学病毒学
结构生物学结构生物学
哺乳动物生物学哺乳动物生物学

背景情况:

严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 感染了各种各样的宿主.
河马,半水生哺乳动物,容易感染SARS-CoV-2.
了解SARS-CoV-2在新宿主中的入侵机制对于监视至关重要.

研究的目的:

为了描述河马血管酶转化酶2 (hiACE2) 与SARS-CoV-2尖端蛋白质的结合.
阐明hiACE2-SARS-CoV-2相互作用的结构基础.
评估海马和相关物种中SARS-CoV-2感染的潜在风险.

主要方法:

对hiACE2与SARS-CoV-2原型 (PT) 和关注的变体 (VOCs) 的结合测试会增加蛋白质受体结合域 (RBDs).
电子显微镜 (cryo-EM) 用于确定与hiACE2复合的SARS-CoV-2 PT S蛋白质的结构.
对ACE2正义学家的突变和比较分析.

主要成果:

hiACE2有效地与SARS-CoV-2 PT和VOC RBDs结合,促进伪病毒的进入.
低温EM检测显示,hiACE2中存在关键残留物L30和F83,是该物种特有的,影响结合亲和力.
结构分析表明,鱼也可能易受SARS-CoV-2感染.

结论:

hiACE2在河马的SARS-CoV-2易感性中发挥着关键作用.
独特的hiACE2残留物有助于产生特定物种的结合特性.
这些发现突显了SARS-CoV-2可能蔓延到半水生和水生哺乳动物的可能性,强调需要加强监测.

关键词:

在ACE2中,ACE2是ACE2.这就是SARS-CoV-2病毒.跨物种识别跨物种识别化-EM结构的结构.河马是一条河马.受体结合域 (RBD) 是一个受体结合域.尖刺 (S) 是一个尖刺.

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