相关实验视频

Updated: Jun 29, 2025

Competitive Homing Assays to Study Gut-tropic T Cell Migration

Competitive Homing Assays to Study Gut-tropic T Cell Migration

Published on: March 1, 2011

克服异抗原免疫力,使免疫能力高的"NoGlow"小鼠能够进行细胞跟踪和基因调控

Timothy N Trotter¹, Andrea Wilson², Jason McBane¹

¹Department of Surgery, Duke University, Durham, North Carolina.

Cancer research communications

|April 9, 2024

概括

我们开发了NoGlow小鼠,表达非功能性外来蛋白质,防止免疫排斥,并使细胞跟踪和基因调节用于生物医学研究.

科学领域:

免疫学免疫学免疫学
遗传学是一个遗传学.
生物医学研究生物医学研究

背景情况:

动物模型中的异生菌基因表达可以触发免疫反应,阻碍细胞跟踪和基因调节.
诱导耐受性或限制表达的现有方法有局限性,例如废除报告员实用性或次优耐受性.

研究的目的:

开发一种新的小鼠模型,克服对外基因的免疫反应,以可靠的细胞跟踪和基因调节.
验证该模型对研究中心和外围耐受性机制的实用性.
为了使瘤学,发育生物学和移植的体内研究.

主要方法:

开发了"NoGlow"小鼠与CRE介导的重组,以表达抗原完好但非功能形式的GFP,Luc和rtTA3.
利用特定组织的CREs来评估免疫耐受性的模型和基于性别的差异.
疫苗接种后评估背景光/发光和适应性免疫力对转基因异原抗原.
证明了三重转基因细胞的跟踪和四环素诱导基因调节.

主要成果:

NoGlow小鼠没有可检测的背景光或发光.
在接种疫苗后观察到对编码的转基因异原抗原的有限适应性免疫力.
NoGlow小鼠成功地跟踪并允许三重转基因细胞的基因调节,与转基因负对照不同.
该模型支持 de novo 转移从正统植入的瘤细胞,尽管高的异抗原表达.

结论:

NoGlow小鼠模型通过克服对外基因的免疫排斥,为体内研究提供了关键资源.
这种模型可以实现可靠的细胞跟踪和四环素诱导基因调节.
在研究适应性免疫,转移,免疫治疗和耐受性方面,NoGlow小鼠非常有价值.

更多相关视频

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Induction of Graft-versus-host Disease and In Vivo T Cell Monitoring Using an MHC-matched Murine Model

Published on: August 29, 2012

Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

Published on: May 23, 2019

相关概念视频

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.