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富含巨细胞的新型功能性长非编码RNAs LRRC75A-AS1和GAPLINC调节极化和天生的免疫反应

Araceli Valverde^1,2, Raza Ali Naqvi^3,4, Afsar R Naqvi^5,6

¹Department of Periodontics, College of Dentistry, University of Illinois Chicago, Chicago, IL, 60612, USA. avalverd@uic.edu.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

|April 9, 2024

概括

巨细胞 (Mφs) 是关键的免疫细胞. 这项研究确定了巨丰富的长非编码RNA (lncRNA),如LRRC75A-AS1和GAPLINC,它们调节Mφ极化和天生的免疫力,在牙周炎中发挥作用.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

巨细胞 (Mφs) 是关键的免疫细胞,弥合了先天性和适应性免疫.
控制Mφ可塑性和功能的表观遗传机制仍然不完全理解.

研究的目的:

确定巨丰富的长非编码RNAs (lncRNAs) 在调节Mφ极化和先天免疫反应中的新功能.
研究特定的lncRNAs在Mφ分化和功能中的作用.

主要方法:

RNA测序 (RNAseq) 用于在M1和M2 Mφs中的 lncRNAs进行分析.
敲除实验以评估LRRC75A-AS1,GAPLINC和AL139099.5对Mφ特征的影响.
流细胞计,光显微镜,多重珠数组和基于PCR的数组用于分析Mφ标记物,细胞化,细胞因子概况和信号通路.
从健康和牙周炎患者的人类牙活检中分析lncRNAs和M1/M2标记物.

主要成果:

数以千计的差异表达的 lncRNAs 在极化 Mφs. 中被确定.
LRRC75A-AS1和GAPLINC的淘汰使Mφ偏向向M2,减少了M1标记物,并影响了细胞化,抗原处理和细胞因子分泌.
这些lncRNAs的淘汰影响了Mφ迁移,通过降低细胞骨信号通路的调节.
在牙周炎中,LRRC75A-AS1和GAPLINC的高调,与M1标志物相关.

结论:

极化巨细胞表现出明显的lncRNA特征,包括新的序列.
LRRC75A-AS1和GAPLINC是Mφ极化和先天免疫功能的关键调节者.
这些lncRNAs在炎症中起着重要作用,并与牙周炎的发病有关.

关键词:

豁免权是一种豁免权.长非编码RNA是什么意思巨细胞的两极分化牙周炎症是一种牙周炎症.发细胞症 (phagocytosis) 是一种致死细胞的发生.

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