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从混合芽中产生一种产生多药耐药扩展谱β-乳糖酶 (ESBL) 的肠杆菌hormaechei菌株
Elizabeth A Leighton1, Chelsea N Gale1, En Huang2
1Department of Microbiology, University of Wisconsin-La Crosse, 1725 State Street, La Crosse, WI, 54601, USA.
Current microbiology
|April 9, 2024
概括
新鲜蔬菜可以携带抗生素耐药的细菌,如扩展光谱β-乳糖酶 (ESBL) 生产的肠杆菌. 这项研究在芽中发现了一种产生ESBL的多药耐药Enterobacter hormaechei菌株,突出显示了与蔬菜相关的抗菌素耐药性 (AR).
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 基因组学就是基因组学.
背景情况:
- 新鲜蔬菜可以成为抗生素耐药细菌的储存库,包括扩展光谱β-乳糖酶 (ESBL) 生产的肠杆菌.
- 肠杆菌hormaechei是一种常见的医院病原体,属于肠杆菌 (Enterobacterales) 类.
研究的目的:
- 为了表征一种多药耐药,产生ESBL的E. hormaechei菌株,从混合芽中分离出来.
- 调查新鲜蔬菜在抗菌素耐药性 (AR) 传播中的作用.
主要方法:
- 使用选择性丰富和ChromagarTM ESBL板块从芽样中分离细菌.
- 通过组合磁盘扩散试验证实ESBL和AmpCβ-lactamase的产生.
- 全基因组测序以识别细菌菌株及其抗生素耐药性 (AR) 基因.
主要成果:
- 一种E. hormaechei菌株 (057-E1) 被从芽中分离出来,并被证实产生ESBL和AmpCβ-lactamases.
- 隔离物对多种抗生素产生了耐药性,包括头素,四环素和三甲-硫甲醇.
- 全基因组测序揭示了多个AR基因的存在,包括blaCTX-M-15基因集群.
结论:
- 新鲜蔬菜在生态和抗生素耐药性传播方面发挥着重要作用.
- 需要有效的策略来缓解新鲜产品中的抗菌素耐药性,以确保食品安全.
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