在酸压力下对Kluyveromyces marxianus的转录组分析和强壮菌株的发展

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Du-Wen Zeng¹, Yong-Qiang Yang², Qi Wang¹

¹Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Applied microbiology and biotechnology

|April 9, 2024

概括

克鲁伊维罗米塞斯马克西安纳斯 (Kluyveromyces marxianus) 是一个植物.

科学领域:

生物技术和合成生物学
微生物生物技术微生物生物技术
工业微生物学工业微生物学

背景情况:

Kluyveromyces marxianus是一种非传统的酵母,具有工业应用的理想特征.
酸 (SA) 是一种有价值的平台化学物质,但其由酵母产生的毒性可能受到限制.
了解SA压力下的基因表达变化对于改善酵母细胞工厂至关重要.

研究的目的:

为了研究K. marxianus暴露于不同度的酸的全球基因转录变化.
为了确定酸响应的促进剂,以加强基因表达控制.
探索改善酵母对酸耐受性的遗传修饰.

主要方法:

在不同度的酸下对K. marxianus的比较转录组分析.
对基因集群的生物信息分析,以确定受影响的代谢途径.
鉴定和验证酸反应的促进剂.
对转录因子Gcr1,Upc2和Ndt80.80的基因过度表达研究.

主要成果:

酸暴露改变了全球基因转录,抑制了三碳酸和氧酸循环,同时激活了糖解和厄戈斯特醇合成途径.
在IMTCP2和KLMA_50231的促进器区域显示显著的激活 (分别为43.4%和154.7%) 在酸15g/L.
转录因子Gcr1,Upc2和Ndt80的过度表达在酸压力下增强了酵母的生长.

结论:

酸显著影响K. marxianus. 的转录格局.
IMTCP2和KLMA_50231促进体是开发SA响应性遗传回路的有希望的目标.
工程Gcr1,Upc2和Ndt80可以提高K. marxianus对于有机酸生产的强度.

关键词:

克鲁伊维罗米塞斯马克西安纳斯 (Kluyveromyces marxianus) 是一个植物.在SA响应基因的SA响应基因.SA 的耐受性 SA 的耐受性.酸 (SA) 是一种酸.对转录组进行分析.

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