药物的化行为在高速逆流色谱中使用与金属离子的柱上复合
Yukiko Moriiwa1, Atsushi Shoji2, Yoichi Shibusawa2
1Department of Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan. moriiwa@toyaku.ac.jp.
概括
这项研究证明了用于药物确定的高速逆流色谱 (HSCCC) 的柱上复杂化. 金属离子复合显著改变药物保留,使含的药物能够被选择性地分离.
科学领域:
- 分析化学 分析化学
- 染色图学技术 染色图学技术
- 协调化学 协调化学
背景情况:
- 高速逆流色谱 (HSCCC) 是一种液-液分离色谱技术.
- 由于具有相似的化学性质,分离含药物可能具有挑战性.
- 在列上复杂化提供了一种新的方法来修改分析物的保留行为.
研究的目的:
- 调查HSCCC中对金属离子的柱上复合的使用,以确定含有的药物.
- 评估金属离子复合对布罗马泽 (BMP) 保留行为的影响.
- 使用HSCCC探索各种含药物与不同金属离子的复合能力.
主要方法:
- 使用高速度逆流色谱 (HSCCC) 采用三甲基乙-乙-水溶剂系统.
- 研究了与铜离子 (CuCl2) 添加和不添加的布罗马泽巴姆 (BMP) 的保留.
- 测试了五种含药物的复杂化 (tzanidine,phentolamine,ambroxol,voriconazole) 与七种金属离子 (Al3+,Fe2+,Co2+,Ni2+,Cu2+,Zn2+) 的复杂化.
主要成果:
- 由于形成极性BMP-Cu2+复合体,布罗马泽 (BMP) 在与铜离子复合时,在HSCCC中显著改变了保留.
- 提扎尼丁和芬托胺形成了多种金属离子 (Al3+,Fe2+,Co2+,Ni2+,Cu2+,Zn2+) 的复合体.
- 阿姆布罗克索尔与Al3+,Fe2+和Cu2+形成复合体,而沃里科纳则没有与任何测试的金属离子形成复合体.
结论:
- 用金属离子在柱上复合是一种有效的策略,可以调节和增强HSCCC中含有的药物的分离.
- 观察到的差异复杂化行为为选择性药物确定和分离提供了基础.
- 这种方法为分析复杂的药物混合物提供了一个有前途的途径.
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