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新的表观遗传修饰剂抑制剂增强面包小麦微胞胎胚胎发生

Isabel Valero-Rubira¹, María Pilar Vallés¹, Begoña Echávarri¹

¹Department of Genetics and Plant Breeding, Aula Dei Experimental Station, Spanish National Research Council (EEAD-CSIC), 50059 Zaragoza, Spain.

Plants (Basel, Switzerland)

|April 9, 2024

概括

新的表观遗传修饰剂增强了植物育种. 光激酶抑制剂II (AUKI-II) 通过增加染色体翻倍,改善了双倍哈普洛伊德 (DH) 植物的生产,特别是在响应较弱的小麦品种中.

科学领域:

植物科学植物科学
农业生物技术农业生物技术
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

双倍平分体 (DH) 技术加速了作物品种的发展.
微胞胚生成 (ME) 是生产DH植物的一个关键过程.
表观遗传修饰剂可以提高ME的效率,但对小麦的有效选择有限.

研究的目的:

选用于改善小麦ME诱导的新型表观遗传修饰剂.
为了评估组织素甲基化和酸化抑制剂的疗效.
确定增加DH植物产量的化合物,特别是在具有挑战性的品种中.

主要方法:

在小麦ME中查素,CARM1抑制剂,极光激酶抑制剂II (AUKI-II) 和hesperadin.
比较AUKI-II和三态素A (TSA) 对胚胎结构形成的影响.
在曼尼托尔压力期间测试AUKI-II的短期和长期应用策略.

主要成果:

柴托辛和AUKI-II增加了胚胎结构;AUKI-II的表现优于TSA.
在长时间的压力中使用AUKI-II增强了染色体的翻倍.
0.8μM AUKI-II在一个中低响应品种中显著增加了绿色DH植物.

结论:

AUKI-II是一种有前途的表观遗传修饰剂,用于增强小麦中的DH植物产量.
这项研究确定了新的表观遗传修饰剂,以改善植物育种中的细胞重编程.
优化AUKI-II的应用可以提高反抗性小麦基因型的DH效率.

关键词:

极光激酶抑制剂II 极光激酶抑制剂II 面包麦子小麦面包一个双倍的平分体.表观遗传修饰剂表观遗传修饰剂微胞子胚胎发生的过程这是自发的染色体倍增.

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