综合转录组和蛋白质组分析显示,细胞壁活动会影响Phelipanche aegyptiaca的寄生
Meixiu Chen1,2, Lu Zhang1,2, Zhaoqun Yao2
1Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization of Xinjiang Production and Construction Corps, Shihezi University, Shihezi 832003, China.
Plants (Basel, Switzerland)
|April 9, 2024
概括
菲利潘希 (Phelipanche aegyptiaca) 是一个古埃及.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 菲利潘希 (Phelipanche aegyptiaca) 导致大量的作物损失.
- 了解它的寄生机制对于控制至关重要.
研究的目的:
- 通过转录组和蛋白质组分析分析Phelipanche aegyptiaca的寄生机制.
- 为了研究特定基因在Phelipanche aegyptiaca寄生病中的作用.
主要方法:
- 对两个Phelipanche aegyptiaca菌株 (HY13M和TE9M) 进行了转录组和蛋白质组分析.
- 使用HIGS (宿主诱导的基因沉默) 的基因沉默被用来研究基因功能.
- 对Citrullus lanatus寄生能力的比较分析.
主要成果:
- TE9M菌株的寄生能力明显强于HY13M菌株.
- 不同表达的基因 (DEGs) 和蛋白质 (DEPs) 在细胞壁代谢途径中得到丰富.
- 沉默TR138070_c0_g1基因,编码细胞壁降解蛋白酶,显著减少Phelipanche aegyptiaca的数量和重量.
结论:
- 细胞壁代谢是Phelipanche aegyptiaca寄生能力和分化的组成部分.
- TR138070_c0_g1基因在Phelipanche aegyptiaca寄生症中发挥着至关重要的作用.
相关概念视频
01:02Role of Microtubules in Cell Wall Deposition
2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K
02:43Plant Cell Wall
56.5K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.5K