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在两种Camellia物种中,FT在花诱导中的作用
Xiong Wang1,2, Jiyuan Li1, Hengfu Yin1
1Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Plants (Basel, Switzerland)
|April 9, 2024
概括
开花的位置T (FT) 基因在Camellia物种中调节开花时间. 在 CaFT 和 CaFT 中.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 遗传学 遗传学 是一个
背景情况:
- 开花地点T (FT) 是植物中开花过渡和花束结构的关键调节者.
- 了解多年生植物中的FT基因功能,特别是关于连续开花的功能,对于作物改进至关重要.
- 以前对FT基因的研究主要集中在一年生植物上,对多年生物种的知识存在差距.
研究的目的:
- 从两个具有鲜明的开花表型的Camellia物种中分离和表征同源的FT基因.
- 研究FT基因在调节卡梅利亚花开时间中的作用背后的分子机制.
- 为了比较一个持续开花的物种 (Camellia azalea) 和一个季节性开花的物种 (Camellia japonica) 的FT基因的功能.
主要方法:
- 来自Camellia azalea和Camellia japonica的FT同源基因 (CaFT和CjFT) 的分离和表征.
- 使用定量实时聚合酶链反应 (qRT-PCR) 在不同组织和不同时间点进行基因表达分析.
- 通过阿拉比多普西斯的过度表达和Camellia azalea中Agrobacterium介导的转化进行功能分析.
- 转录组分析以确定FT调节的下游和上游基因.
主要成果:
- 两个同源的FT基因,CaFT和CjFT被分离和特征化,CjFT拥有额外的五氨基酸重复序列.
- FT基因在叶子和花中表现出明显的表达模式,C. azalea中CaFT表现出全年表达,与C. japonica.中的CjFT不同.
- 在Arabidopsis中CaFT和CjFT的过度表达促进了开花和上调下游开花基因 (AP1,FUL,SEP3) 和稍微上调上游基因 (CO,GI).
结论:
- 两种CaFT和CjFT基因都在促进卡梅利亚物种的开花中发挥作用.
- 在C. azalea叶子中CaFT全年表达模式可能与其连续开花的表型有关.
- FT基因充当花源,通过调节下游和上游的开花路径基因来调节开花.
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