朗格亚病毒糖蛋白抗原的结构和设计
Zhaoqian Wang1, Matthew McCallum1, Lianying Yan2
1Department of Biochemistry, University of Washington, Seattle, WA 98195.
概括
兰雅病毒 (LayV) 融合和附着糖蛋白通过新型受体介导细胞进入. 针对相关henipaviruses的抗体不会中和LayV,因此需要开发新的疫苗和治疗方法.
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 免疫学 免疫学 免疫学
背景情况:
- 朗格亚病毒 (LayV) 是一种在中国发现的新型亨尼帕病毒 (HNV).
- 包括尼帕病毒 (NiV) 和亨德拉病毒 (HeV) 在内的HNV利用G和F糖蛋白进入宿主细胞.
- 这些葡萄糖蛋白是中和抗体的主要点.
研究的目的:
- 为了描述LayV糖蛋白的细胞进入机制.
- 为了研究LayV F和G糖蛋白的抗原性.
- 探索开发特定于LayV的疫苗和治疗方法的战略.
主要方法:
- 确定了LayV F (融合前和融合后状态) 和G糖蛋白的冷电子显微镜结构.
- 评估LayV糖蛋白介导膜融合与各种宿主细胞.
- 评估了NiV和HeV特异性抗体与LayV糖蛋白的交叉活性.
- 通过计算设计稳定LayV G结构.
主要成果:
- 与NiV和HeV相比,LayV F和G糖蛋白通过不同的受体进行介导膜融合,而NiV和HeV则使用不同的受体.
- 针对NiV和HeV的单克隆和多克隆抗体与LayV F和G没有交叉反应.
- 低温EM结构揭示了LayV糖蛋白的构造格局和独特的抗原性.
- 一种稳定HNV F糖蛋白的策略被证明是可通用的.
结论:
- 莱维采用独特的细胞进入机制,与其他HNV不同.
- 现有的HNV抗体对LayV无效.
- 对LayV糖蛋白的结构洞察对于开发向疫苗和治疗方法至关重要.
- 对HNV糖蛋白的稳定策略显示出更广泛的应用的希望.
相关概念视频
Viral Structure
62.2K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.2K
Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Glycocalyx and its Functions
4.0K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
4.0K


