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Yuta Michimori^1,2, Rikihisa Izaki¹, Yu Su¹

¹Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.

Proceedings of the National Academy of Sciences of the United States of America

|April 9, 2024

概括

超热友的archaeon Thermococcus kodakarensis 清除了2-糖酸盐,这是鲁比斯科活动的副产品,独立于卡尔文-本森-巴什姆循环. 这种生物分泌糖,这表明了一种新的光呼吸类似过程的途径.

科学领域:

生物化学生物化学
微生物学微生物学
考古物 (Archaea) 是一种古老的物种.

背景情况:

使用Calvin-Benson-Bassham (CBB) 循环的生物通常具有管理2-糖酸盐的途径,这是Rubisco氧化酶活动的副产品.
C2途径通常被认为是CBB循环生物和氧光合作用中的2-糖酸代谢和氧光合作用的组成部分.
超热友的古生物学家*Thermococcus kodakarensis*在酸二酸盐路径中使用Rubisco,而不是CBB循环.

研究的目的:

为了研究2糖酸盐去除和救援的机制在*Thermococcus kodakarensis*.
为了确定这个archaeon中的2-糖酸盐代谢是否与CBB循环有关,还是独立运行.

主要方法:

生物化学测试以确定参与2-糖酸盐转化中的酶.
基因分析,包括基因破坏,以评估已识别的酶的功能.
在不同氧气条件下的生长实验 (无氧与微氧) 和分泌代谢物的分析.

主要成果:

在 *T. kodakarensis* 中发现了能够将2-糖酸转化为甘氨酸和氨酸的酶.
破坏TK1734基因,编码特定的2-糖酸酸酶,在微空气条件下增长受损.
微空气培养的细胞分泌了糖酸盐,并且通过添加硫化来挽救生长缺陷.

结论:

*T. kodakarensis* 具有功能性的2-糖酸酶 (TK1734),在微空气条件下对生长至关重要.
考古团似乎通过脱化和随后的糖分泌来去除2-糖酸盐.
这些发现表明,2-糖酸盐代谢可以独立于CBB循环发生,这挑战了以前的假设.

关键词:

这是一种2-糖酸盐.考古物 (Archaea) 是一种古老的物种.鲁比斯科 (Rubisco) 是一个古老的葡萄酒.代谢过程中的代谢.

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