布朗运动为纳米孔中的分子转位铺平了道路
Won-Yong Lee1, Chenyu Wen2,3, Ngan Hoang Pham1
1Division of Solid-State Electronics, Department of Electrical Engineering, Uppsala University, Uppsala, 75103, Sweden.
Small methods
|April 9, 2024
概括
一个新的模拟模型有助于分析纳米孔转移事件,克服高频测量中的噪声挑战. 它有助于辨别事件并优化固态纳米孔传感器设计.
科学领域:
- 纳米技术 纳米技术
- 生物物理学的生物物理.
- 分析化学 分析化学
背景情况:
- 高频测量对于跟踪快速纳米孔转移分析剂至关重要.
- 增加采样带宽面临高频噪声带来的挑战,影响事件捕获的准确性.
研究的目的:
- 呈现一个数值模拟模型作为辨别纳米孔转移事件的替代方案.
- 分析各种实验参数对分析物转位的影响.
主要方法:
- 开发了一个数值模拟模型来追踪纳米孔内的分析物轨迹.
- 在转位过程中对分析物起作用的模拟力 (布罗恩力,电宇宙力).
- 各种参数:孔径尺寸,偏向电压,分析物电荷,盐度,电解质粘度.
主要成果:
- 布朗力主导着分析物的运动,直到电宇宙力决定了最终的转移.
- 斯特维丁类分析物的平均停留时间从~6μs降至~1μs,偏差电压增加 (±100~±500mV).
- 模拟事件显示与实验性链毒素数据的定性一致.
结论:
- 模拟模型有效地识别转位事件,并有助于理解所涉及的力量.
- 该模型对于设计改进的固态纳米孔传感器非常有价值.
- 它为高频测量中的实验限制提供了替代方案.
更多相关视频
相关概念视频
Protein Diffusion in the Membrane
4.3K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.3K
Facilitated Diffusion
452
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
452
Aquaporins
4.8K
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
4.8K
Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
Protein Dynamics in Living Cells
2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K


