一种甲基转移酶AflIme4调节细胞的生长,发育和阿斯伯吉路斯黄菌中的亚拉托克辛B生物合成
Liuke Liang1, Xiaoyan Wang1, Shan Wei1
1College of biological engineering, Henan University of Technology, Zhengzhou 450001, China.
Microbiological research
|April 9, 2024
概括
一种新型的酶AflIme4调节阿斯伯吉路斯黄菌的mRNA甲基化,显著降低了阿弗拉托克辛B1 (AFB1) 的产生,并影响了真菌的生长. 这一发现为控制AFB1污染提供了一个潜在的目标.
科学领域:
- 分子生物学分子生物学
- 菌类学 菌类学是指菌类学.
- 食品安全 食品安全
背景情况:
- 由Aspergillus flavus产生的亚毒素B1 (AFB1) 对农业,食品安全和人类健康构成重大威胁.
- 众所周知,信使RNA (mRNA) N6-甲基氨酸 (m6A) 甲基化调节了A. flavus的生长和AFB1的产生,但它的特定作用需要进一步调查.
研究的目的:
- 在A. flavus.中识别和表征一种新的m6A甲基转移酶,AflIme4.
- 阐明AflIme4在调节真菌发育和AFB1生物合成中的作用.
主要方法:
- 鉴定和描述AflIme4基因及其蛋白质产物.
- 产生A. flavus ΔAflIme4淘汰菌株.
- 分析真菌生长,发育和AFB1产生的情况.
- 用于基因表达分析的RNA测序 (RNA-Seq) 和RT-qPCR.
- 甲基化RNA免疫沉降-qPCR (MeRIP-qPCR) 来评估m6A修饰水平和mRNA稳定性.
主要成果:
- AflIme4局部存在于细胞质中,其缺乏显著降低了整体mRNA甲基化.
- AflIme4的淘汰损害了A. flavus的生长,结合,疏水性,结产量和致病性,同时增加了对氧化和透应激的敏感性.
- 最重要的是,在 ΔAflIme4 菌株中,AFB1 生产明显受抑制.
- RNA-Seq和MeRIP-qPCR发现,AflIme4增强了AFB1生物合成途径中的m6A基因甲基化,降低了它们的mRNA稳定性,从而降低了AFB1的产生.
结论:
- AflIme4作为m6A甲基转移酶起作用,减弱AFB1生物合成基因的mRNA稳定性,从而抑制AFB1的产生.
- AflIme4是开发控制A. flavus生长和减轻农业和食品中偏毒素污染的战略的潜在分子标.
更多相关视频
09:21Inhibition of Aspergillus flavus Growth and Aflatoxin Production in Transgenic Maize Expressing the α-amylase Inhibitor from Lablab purpureus L.
Published on: February 15, 2019
10.5K
10:33Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
Published on: May 27, 2022
5.5K
相关概念视频
01:29Fungal Group Zygomycota
Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
01:28Fungal Phylum Ascomycota
Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
01:26Fungal Phylum Basidiomycota
Basidiomycota is a diverse phylum of fungi that includes ecologically significant decomposers such as white rot fungi, symbionts like mycorrhizal fungi, plant pathogens such as rusts and smuts, and edible species like Agaricus bisporus (the common button mushroom). These fungi play crucial roles in nutrient cycling, symbiotic relationships, and even human health. Their defining feature is the basidium, a microscopic club-shaped structure responsible for producing basidiospores.Fruiting Bodies...
01:22Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
01:15Antifungal Agents
Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...