相关实验视频

Updated: Jun 28, 2025

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Determining the Toxicity of UV Radiation and Chemicals on Primary and Immortalized Human Corneal Epithelial Cells

Published on: July 22, 2021

揭示UVB效应:角层中catalase活性和分子变化

Nikol Labecka¹, Michal Szczepanczyk², Enamul Mojumdar³

¹Department of Biomedical Science, Malmö University, SE-205 06 Malmö, Sweden; Biofilms Research Center for Biointerfaces, Malmö University, SE-205 06 Malmö, Sweden; Division of Physical Chemistry, Chemistry Department, Lund University, SE-221 00 Lund, Sweden.

Journal of colloid and interface science

|April 9, 2024

概括

紫外线B (UVB) 辐射使皮肤关键的酶酶失活.

科学领域:

皮肤的屏障功能是皮肤的屏障功能.
照相损伤照相损伤
酶学是一种酶学.

背景情况:

紫外线B (UVB) 辐射通过各种机制破坏皮肤屏障.
角层 (SC) 含有催化酶,一种清除活性氧物种的酶.
紫外线对SC催化酶活性和SC矩阵结构的影响尚未完全理解.

研究的目的:

调查UVB暴露对SC内的催化酶活性和结构的影响.
分析紫外线B诱导的分子动力学和SC质和脂质组织的变化.
描述UVB对SC矩阵及其抗氧化防御机制的影响.

主要方法:

时光度测量用于测量SC中的原生催化酶活性.
循环二重化和凝电泳评估了催化酶结构和碎片化.
固态NMR,X射线衍射,FTIR光谱和动态蒸汽吸附分析了SC的分子动态和结构.

主要成果:

紫外线暴露在SC矩阵和冷化形式中的催化酶被禁用,而不会改变其二次结构或引起碎片化.
紫外线辐射诱导了SC脂质和质分子动态的微妙变化,增加了脂质末端段的移动性和质脊柱的刚性.
FTIR数据显示了SC脂质和蛋白质成分的变化,独立于水化能力.

结论:

紫外线辐射会损害皮肤角层中的催化酶功能,可能增加氧化应激.
在SC脂质和蛋白质分子特征的微妙变化发生在UVB暴露后.
这些综合作用可能会损害皮肤健康,增加对皮肤疾病的易感性.

关键词:

催化酶是一种催化剂.酸纤维是酸纤维的纤维.脂质多层质矩阵是多层质的.氧化应激是一种氧化应激.角质层 (stratum corneum) 是一个层.紫外线 (UVB) 是一种UVB.紫外线辐射的紫外线辐射.

更多相关视频

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C UV-C Damage

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C UV-C Damage

Published on: February 15, 2020

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Characterization of Molecular Mechanisms of In vivo UVR Induced Cataract

Published on: November 28, 2012

相关概念视频

Mutations

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...

Nucleotide Excision Repair

Nucleotide Excision Repair

Skin Cancer

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...