相关实验视频

Updated: Jun 28, 2025

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies

Published on: October 6, 2022

通过TrkC介导的DRG神经元中CCL2升高,NT-3有助于化疗诱导的神经病痛

Dilip Sharma¹, Xiaozhou Feng¹, Bing Wang¹

¹Department of Anesthesiology, New Jersey Medical School, Rutgers, The State University of New Jersey, Newark, NJ, 07103, USA.

|April 9, 2024

概括

在背部根系神经元中,神经营养素-3 (NT3) 的上调有助于化疗诱导的神经病痛 (CINP). 阻断NT3可以缓解疼痛,这表明NT3是CINP管理的潜在治疗点.

科学领域:

神经科学是一个神经科学.
疼痛研究疼痛研究
在瘤学瘤学.

背景情况:

化疗诱导的神经病痛 (CINP) 是抗瘤药物治疗的常见和衰弱的副作用.
目前用于管理CINP的治疗选择有限,这突显了对新型治疗策略的需求.

研究的目的:

调查神经热素-3 (NT3) 在帕克利塔塞尔诱导的神经病痛的发展中的作用.
阐明NT3对CINP作出贡献的潜在分子机制.

主要方法:

在雄性和雌性小鼠中,系统注射帕克利塔塞尔来诱导神经病痛.
在背部根 (DRG) 和脊髓中评估NT3mRNA和蛋白质表达.
药理封锁或模仿NT3的上调.
对 nociceptive 过敏和自发疼痛行为进行评估.
对热氨酸受体激酶C (TrkC) 激活和C-C化学因子联结体2 (Ccl2) 表达的分析.

主要成果:

帕克利塔克塞尔治疗提高了DRG神经元中的NT3表达,但在脊髓中没有.
阻断NT3上调减弱了帕克利塔塞尔诱导的机械,热和冷过敏以及自发疼痛.
在天真小鼠中模仿NT3增加增强了疼痛反应.
TrkC的NT3激活与DRG中帕克利塔塞尔诱导的Ccl2增加有关.

结论:

NT3在化疗诱导的神经病痛的发展中起着重要作用.
在DRG神经元中,NT3通过TrkC介导的CCL2激活,对CINP作出贡献.
NT3代表了缓解CINP的潜在治疗标.

关键词:

CC 化学基因连接物 2 连接物背部根系关节背部根系神经营养素-3是一种神经营养素.帕克利塔塞尔诱导的外周神经病变 TrkCC 的意思是什么?

更多相关视频

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1

Published on: February 13, 2018

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

The Sciatic Nerve Cuffing Model of Neuropathic Pain in Mice

Published on: July 16, 2014

相关概念视频

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy. SP binds and activates...

Analgesia and Pain Management

Analgesia and Pain Management

Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...